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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biomass Accumulation and Cell Wall Structure of Rice Plants Overexpressing a Dirigent-Jacalin of Sugarcane (ShDJ) Under Varying Conditions of Water Availability

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Andrade, Larissa Mara [1, 2] ; Peixoto-Junior, Rafael Favero [1, 2] ; Ribeiro, Rafael Vasconcelos [3] ; Nobile, Paula Macedo [1] ; Brito, Michael Santos [4, 1] ; Ribeiro Marchiori, Paulo Eduardo [5, 6] ; Carlin, Samira Domingues [1] ; Boer Martins, Alexandre Palma [1, 2] ; Goldman, Maria Helena S. [7] ; Portilla Llerena, Juan Pablo [3] ; Fregonesi, Caroline [1] ; Perecin, Dilermando [8] ; Carlos de Oliveira Nebo, Joao Felipe [9] ; Figueira, Antonio [9] ; Benatti, Thiago Romanos [10] ; da Silva, Jorge [10] ; Mazzafera, Paulo [3] ; Creste, Silvana [1]
Total Authors: 18
Affiliation:
[1] Ctr Cana, Inst Agron IAC, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, PPG Genet, Ribeirao Preto - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, Campinas, SP - Brazil
[4] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Sao Jose Dos Campos - Brazil
[5] Univ Fed Lavras, Dept Biol, Lavras - Brazil
[6] Ctr Ecofisiol & Biofis, Inst Agron IAC, Campinas, SP - Brazil
[7] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto - Brazil
[8] Univ Estadual Paulista Julio de Mesquita Filho Un, Fac Ciencias Agr & Vet, Jaboticabal - Brazil
[9] Univ Sao Paulo, CENA, Piracicaba - Brazil
[10] Texas A&M Agrilife Res & Extens Ctr, Weslaco, TX - USA
Total Affiliations: 10
Document type: Journal article
Source: FRONTIERS IN PLANT SCIENCE; v. 10, FEB 13 2019.
Web of Science Citations: 0
Abstract

A sugarcane gene encoding a dirigent-jacalin, ShDJ, was induced under drought stress. To elucidate its biological function, we integrated a ShDJ-overexpression construction into the rice Nipponbare genome via Agrobacterium- mediated transformation. Two transgenic lines with a single copy gene in T-0 were selected and evaluated in both the T-1 and T-4 generations. Transgenic lines had drastically improved survival rate under water deficit conditions, at rates close to 100%, while WT did not survive. Besides, transgenic lines had improved biomass production and higher tillering under water deficit conditions compared with WT plants. Reduced pectin and hemicellulose contents were observed in transgenic lines compared with wild-type plants under both well-watered and water deficit conditions, whereas cellulose content was unchanged in line \#17 and reduced in line \#29 under conditions of low water availability. Changes in lignin content under water deficit were only observed in line \#17. However, improvements in saccharification were found in both transgenic lines along with changes in the expression of OsNTS1/2 and OsMYB58/63 secondary cell wall biosynthesis genes. ShDJ-overexpression upregulated the expression of the OsbZIP23, OsGRAS23, OsP5CS, and OsLea3 genes in rice stems under well-watered conditions. Taken together, our data suggest that ShDJ has the potential for improving drought tolerance, plant biomass accumulation, and saccharification efficiency. (AU)

FAPESP's process: 17/24420-0 - Transgenic sugarcane: addressing an improvement sustainable productivity
Grantee:Silvana Aparecida Creste Dias de Souza
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 08/57495-3 - Integrating physiological, morphological and anatomical traits to understand the differential sucrose yield in sugarcane genotypes
Grantee:Eduardo Caruso Machado
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 11/50661-8 - Characterization of sugar cane transcripts that respond to water stress in order to obtain drought-tolerant transgenic plants
Grantee:Silvana Aparecida Creste Dias de Souza
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants