Daily Consumption of Orange Juice from Citrus sinensis L. Osbeck cv. Cara Cara and cv. Bahia Differently Affects Gut Microbiota Profiling as Unveiled by an Integrated Meta-Omits Approach

Author(s)

Brasili, Elisa ^{[1, 2]} ; Aymoto Hassimotto, Neuza Mariko ^{[1, 2]} ; Del Chierico, Federica ^[3] ; Marini, Federico ^[4] ; Quagliariello, Andrea ^[3] ; Sciubba, Fabio ^[4] ; Miccheli, Alfredo ^[4] ; Putignani, Lorenza ^{[3, 5]} ; Lajolo, Franco ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Food Sci & Expt Nutr, BR-05508000 Sao Paulo - Brazil

^[2] CEPID FAPESP Res Innovat & Disseminat Ctr Sao Pau, Food Res Ctr FoRC, BR-05468901 Sao Paulo - Brazil

^[3] Childrens Hosp & Res Inst Bambino Gesu, Unit Human Microbiome, I-00165 Rome - Italy

^[4] Univ Roma La Sapienza, Dept Chem, I-00185 Rome - Italy

^[5] Childrens Hosp & Res Inst Bambino Gesu, Unit Parasitol, I-00165 Rome - Italy

• Document type: Journal article
• Source: Journal of Agricultural and Food Chemistry; v. 67, n. 5, p. 1381-1391, FEB 6 2019.
• Web of Science Citations: 3

Abstract

We have investigated the effect of intake of two different orange juices from Citrus sinensis cv. ``Cara Cara{''} and cv. ``Bahia{''} on faecal microbiota and metabolome using an integrated meta-omits approach. Following a randomized crossover design, healthy subjects daily consumed 500 mL of orange juice from Cara Cara or Bahia juices or an isocaloric control drink. Stools were collected at baseline (T0) and after a week (T7) of intervention. Operational taxonomic units (OTUs) were pyrosequenced targeting 16S rRNA, and faecal metabolites were analyzed by an untargeted metabolomics approach based on H-1 NMR spectroscopy. The major shift observed in microbiota composition after orange juice intake was the increased abundance of a network of Clostridia OTUs from Mogibacteriaceae, Tissierellaceae, Veillonellaceae, Odoribacteraceae, and Ruminococcaceae families, whose members were differently affected by Cara Cara or Bahia juice consumption. A core of six metabolites such as inositol, choline, lysine, arginine, urocanic acid, and formate significantly increased in Cara Cara compared to the Bahia group. (AU)

• FAPESP's process: 16/08796-7 - Metabolomic approach: a comparative study of orange juices variety 'Cara Cara' and variety 'Bahia' intake in healthy humans
• Grantee: Elisa Brasili
• Support Opportunities: Scholarships in Brazil - Post-Doctorate