| Full text | |
| Author(s): |
de Sousa, J. P.
[1]
;
Carvalho, R. G.
[2]
;
Barbosa-Martins, L. F.
[1]
;
Torquato, R. J. S.
[2]
;
Mugnol, K. C. U.
[3]
;
Nascimento, F. D.
[3]
;
Tersariol, I. L. S.
[2]
;
Puppin-Rontani, R. M.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Pediat Dent, Campinas, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo, SP - Brazil
[3] Univ Mogi das Cruzes, Interdisciplinary Ctr Biochem Invest, BR-08773520 Mogi Das Cruzes, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | JOURNAL OF DENTAL RESEARCH; v. 98, n. 3, p. 347-354, MAR 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
The major goal in restorative dentistry is to develop a true regenerative approach that fully recovers hydroxyapatite crystals within the caries lesion. Recently, a rationally designed self-assembling peptide P-11-4 (Ace-QQRFEWEFEQQ-NH2) has been developed to enhance remineralization on initial caries lesions, yet its applicability on dentin tissues remains unclear. Thus, the present study investigated the interaction of P-11-4 with the organic dentin components as well as the effect of P-11-4 on the proteolytic activity, mechanical properties of the bonding interface, and nanoleakage evaluation to artificial caries-affected dentin. Surface plasmon resonance and atomic force microscopy indicated that P-11-4 binds to collagen type I fibers, increasing their width from 214 +/- 4 nm to 308 +/- 5 nm (P < 0.0001). P-11-4 also increased the resistance of collagen type I fibers against the proteolytic activity of collagenases. The immediate treatment of artificial caries-affected dentin with P-11-4 enhanced the microtensile bonding strength of the bonding interface (P < 0.0001), reaching values close to sound dentin and decreasing the proteolytic activity at the hybrid layer; however, such effects decreased after 6 mo of water storage (P < 0.05). In conclusion, P-11-4 interacts with collagen type I, increasing the resistance of collagen fibers to proteolysis, and improves stability of the hybrid layer formed by artificial caries-affected dentin. (AU) | |
| FAPESP's process: | 13/05822-9 - Proteinase-Activated Receptors (PARs) in the dentin-pulp complex: identification, modulation and signal tranduction in caries disease |
| Grantee: | Fábio Dupart Nascimento |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 14/22899-8 - Effect of remineralizing agents on proteolytic activity of caries-affected dentin and on and resin/dentin bond |
| Grantee: | Jossaria Pereira de Sousa |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 15/03964-6 - Glycosaminoglycans and proteoglycans: interplay between structure and function |
| Grantee: | Helena Bonciani Nader |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/12660-0 - Effect of remineralizing agents on proteolytic activity and resin/caries-affected dentin bond longevity |
| Grantee: | Regina Maria Puppin Rontani |
| Support Opportunities: | Regular Research Grants |