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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

An alkaline active feruloyl-CoA synthetase from soil metagenome as a potential key enzyme for lignin valorization strategies

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Author(s):
Sodre, Victoria [1, 2] ; Araujo, Juscemacia Nascimento [3] ; Goncalves, Thiago Augusto [1, 4] ; Vilela, Nathalia [1, 4] ; Kimus Braz, Antonio Sergio [3] ; Franco, Telma Teixeira [2] ; Neto, Mario de Oliveira [5] ; de Lima Damasio, Andre Ricardo [1] ; Garcia, Wanius [3] ; Squina, Fabio Marcio [4, 2]
Total Authors: 10
Affiliation:
[1] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Fac Chem Engn, Campinas, SP - Brazil
[3] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[4] Univ Sorocaba UNISO, Programa Proc Tecnol & Ambientais, Sorocaba, SP - Brazil
[5] Univ Estadual Paulista UNESP, Inst Biociencias, Dept Fis & Biofis, Botucatu, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: PLoS One; v. 14, n. 2 FEB 25 2019.
Web of Science Citations: 1
Abstract

Ferulic acid (FA), a low-molecular weight aromatic compound derived from lignin, represents a high-value molecule, used for applications in the cosmetic and pharmaceutical industries. FA can be further enzymatically converted in other commercially interesting molecules, such as vanillin and bioplastics. In several organisms, these transformations often start with a common step of FA activation via CoA-thioesterification, catalyzed by feruloyl-CoA synthetases (Fcs). In this context, these enzymes are of biotechnological interest for conversion of lignin-derived FA into high value chemicals. In this study, we describe the first structural characterization of a prokaryotic Fcs, named FCS1, isolated from a lignin-degrading microbial consortium. The FCS1 optimum pH and temperature were 9 and 37 degrees C, respectively, with Km of 0.12 mM and Vmax of 36.82 U/mg. The circular dichroism spectra indicated a notable secondary structure stability at alkaline pH values and high temperatures. This secondary structure stability corroborates the activity data, which remains high until pH 9. The Small Angle X-Ray Scattering analyses resulted on the tertiary/quaternary structure and the low-resolution envelope in solution of FCS1, which was modeled as a homodimer using the hyperthermophilic nucleoside diphosphate-forming acetyl-CoA synthetase from Candidatus Korachaeum cryptofilum. This study contributes to the field of research by establishing the first biophysical and structural characterization for Fcs, and our data may be used for comparison against novel enzymes of this class that to be studied in the future. (AU)

FAPESP's process: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks
Grantee:Telma Teixeira Franco
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/16976-8 - Biophysical and biochemical characterization of two bacterial enzymes involved in the conversion of ferulic acid to vanillin
Grantee:Juscemácia Nascimento Araujo
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/17275-3 - Studies of the mode of action of two Lytic Polysaccharide Monooxygenases from insect (order: Isoptera): molecular structure, bioinorganic chemistry and biotechnological applications
Grantee:Wanius José Garcia da Silva
Support type: Regular Research Grants
FAPESP's process: 18/18101-1 - Development of the CRISPR/Cas9 system in Rhodococcus jostii RHA1 for production of high-value chemicals from lignin streams
Grantee:Victoria Ramos Sodré de Castro
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 17/05901-7 - Development of E. coli-Rhodococcus CRISPR/Cas9 shuttle vector targeting ferulic acid accumulating Rhodococcus jostii RHA1 mutants
Grantee:Victoria Ramos Sodré de Castro
Support type: Scholarships in Brazil - Master
FAPESP's process: 17/22669-0 - N-glycosylation and enzymes secretion in filamentous fungi
Grantee:André Ricardo de Lima Damasio
Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 17/08166-6 - Genetic engineering of yeast for conversion lignocellulosic material to ferulic acid
Grantee:Nathália Vilela
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via feluric acid to high value chemicals
Grantee:Fábio Márcio Squina
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 17/16089-1 - Production of lignin chains, metagenomic screening and biocatalytic conversion of lignin to ferulic acid
Grantee:Thiago Augusto Gonçalves
Support type: Scholarships in Brazil - Post-Doctorate