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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males

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Author(s):
Branco, Giovana Souza [1, 2] ; Melo, Aline Gomes [1] ; Ricci, Juliana M. B. [1] ; Digmayer, Melanie [1] ; de Jesus, Lazaro W. O. [3] ; Habibi, Hamid R. [4] ; Nobrega, Rafael Henrique [1]
Total Authors: 7
Affiliation:
[1] Scio Paulo State Univ UNESP, Inst Biosci, Dept Morphol, Reprod & Mol Biol Grp, Botucatu Campus, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sao Paulo State Univ CAUNESP, Aquaculture Ctr, Jaboticabal Campus, Jaboticabal - Brazil
[3] Fed Univ Alagoas AC, Inst Biol Sci & Hlth, Simoes Campus, Maceio - Brazil
[4] Univ Calgary, Dept Biol Sci, Calgary, AB - Canada
Total Affiliations: 4
Document type: Journal article
Source: General and Comparative Endocrinology; v. 273, n. SI, p. 209-217, MAR 1 2019.
Web of Science Citations: 3
Abstract

The pituitary gonadotropins, Fsh (follicle-stimulating hormone) and Lh (luteinizing hormone), regulate testicular development and functions in all vertebrates. At the pituitary, different signaling systems regulate the synthesis and secretion of the gonadotropins, such as the hypothalamic neuropeptides GnRH (gonadotropin-releasing hormone) and GnIH (gonadotropin-inhibitory hormone). While GnRH exerts stimulatory roles, the actions of GnIH remain controversial for many teleost species. Therefore, the aim of this study was to evaluate the in vitro effects of chicken GnRH2 (cGnRH2) and zebrafish GnIH-3 (zGnIH-3) on the male gonadotropin and GnRH system expression using pituitary explants and brain slices from a neotropical species with economical and ecological relevance, Astyanax altiparanae. Our results showed that in males, cGnRH2 increased fshb and lhb mRNA levels in the pituitary explants. Interestingly, zGnIH-3 has no effect on basal gonadotropin expression, however zGnIH-3 decreased the cGnRH2-induced fshb and lhb transcripts in male pituitary explants. In the male brain slices, zGnIH-3 showed stimulatory effects, increasing gnrh2 mRNA levels. Overall, our results suggested that GnIH seems to have dual regulatory actions on gonadotropin and GnRH2 expression of A. altiparanae males. This study provided basic information on endocrine regulation of A. altiparanae reproduction, and the obtained results will expand our knowledge, improving the reproductive management of this economically important freshwater species. (AU)

FAPESP's process: 15/12104-0 - Effects of cortisol in zebrafish spermatogenesis as mean to understand the role of this steroid during thermal stress induced sex differentiation
Grantee:Aline Gomes Melo
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/07620-7 - Spermatogonial stem cell bank to preserve the genotype of endangered and valuable teleost species
Grantee:Rafael Henrique Nóbrega
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/20450-0 - Molecular markers and cryopreservation of spermatogonial stem cells of common carp, Cyprinus carpio and zebrafish, Danio rerio
Grantee:Melanie Digmayer
Support type: Scholarships in Brazil - Post-Doctorate