Testis structure, spermatogonial niche and Sertoli cell efficiency in Neotropical fish

Author(s)

de Siqueira-Silva, Diogenes Henrique ^[1] ; Rodrigues, Maira da Silva ^{[2, 3]} ; Nobrega, Rafael Henrique ^[2]

Affiliation

^[1] Univ South & Southern Para Unifesspa, PPG Biodivers & Biotechnol BIONORTE, Grp Studies Reprod Amazon Fish GERPA LANEC, Maraba, Para - Brazil

^[2] Selo Paulo State Univ UNESP, Inst Biosci, Dept Morphol, Reprod & Mol Biol Grp, Botucatu Campus, Botucatu, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Aquaculture Ctr Sao Paulo State Univ CAUNESP, Jaboticabal Campus, Jaboticabal - Brazil

• Document type: Journal article
• Source: General and Comparative Endocrinology; v. 273, n. SI, p. 218-226, MAR 1 2019.
• Web of Science Citations: 0

Abstract

Neotropical icthyofauna represents one of the most diverse and extreme ecosystems in the world. Likewise, reproduction showed enormous diversity with different reproductive systems, modes and behavior. On the other hand, information on Neotropical fish species, in particular on male reproductive physiology is restricted to few species. This mini-review aimed to compile the existing information on spermatogenesis of Neotropical teleosts focusing on testis structure, spermatogonial niche and Sertoli cell efficiency. The first topic covers the histological analysis of the testicular structure, showing a conserved testicular pattern in relation to the phylogenetic position: basal species present anastomosing tubular testis (e.g. Astyanax altiparanae, Conorhynchos conirostris, Pimelodus maculatus, Lophiositurus alexandri, Rhinelepis aspera, among others), while derived teleosts showed lobular testis (e.g. Cichlasoma dimerus, Cichla kelberi, Odontesthes bonariensis, Synbranchus marmoratus and others). Next to testicular structure, existing data showed that type A undifferentiated spermatogonia (A(und)) is differentially distributed among the Neotropical species. A(und) can be restricted at the blind-end of the germinal compartment (O. bonariensis), or spread along the germinal epithelium (A. altiparanae), or even distributed along the germinal epithelium but concentrated at the blind-end (C. kelberi and C. intermedia). Moreover, recent studies in A. altiparanae have demonstrated that within the germinal compartment, A(und) have a preferential distribution in areas neighboring the interstitial compartment - the spermatogonial niche. The proximity with the interstitium suggests that interstitial cells, such as Leydig cells, are important for A(und) maintenance in the testis. Finally, this mini-review highlighted Sertoli cell efficiency, showing that a single Sertoli cell can support a higher number of germ cells (80-140 spermatids) in Neotropical species evaluated at the moment (e.g. A. altiparanae, Hoplias malabaricus, Poecilia reticulata, Serrasaltnus spilopleura, C. intermedia). Overall, this review provided basic and functional information on spermatogenesis of Neotropical species. More studies in this field are necessary since Neotropical region is considered one of the hotspot regions to discovery new species providing, therefore, new opportunities to investigate spermatogenesis in fish. (AU)

• FAPESP's process: 14/07620-7 - Spermatogonial stem cell bank to preserve the genotype of endangered and valuable teleost species
• Grantee: Rafael Henrique Nóbrega
• Support type: Research Grants - Young Investigators Grants
• FAPESP's process: 17/15793-7 - Effects of hypothyroidism on testicular function of Danio rerio and relation between thyroid hormones and follicle stimulating hormone (Fsh)
• Grantee: Maira da Silva Rodrigues
• Support type: Scholarships in Brazil - Master