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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Duration of spermatogenesis and identification of spermatogonial stem cell markers in a Neotropical catfish, Jundia (Rhamdia quelen)

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Author(s):
Lacerda, S. M. S. N. [1] ; Martinez, E. R. M. [2] ; Mura, I. L. D. D. [2] ; Doretto, L. B. [2] ; Costa, G. M. J. [1] ; Silva, M. A. [1] ; Digmayer, M. [2] ; Nobrega, R. H. [2] ; Franca, L. R. [1, 3]
Total Authors: 9
Affiliation:
[1] Univ Fed Minas Gerais, Inst Biol Sci, Dept Morphol, Lab Cellular Biol, Belo Horizonte, MG - Brazil
[2] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, Reprod & Mol Biol Grp, Botucatu, SP - Brazil
[3] Natl Inst Amazonian Res, Manaus, AM - Brazil
Total Affiliations: 3
Document type: Journal article
Source: General and Comparative Endocrinology; v. 273, n. SI, p. 249-259, MAR 1 2019.
Web of Science Citations: 0
Abstract

Spermatogenesis is a process driven by stem cell, where germ cell cycle is under the control of a specific genotype species. Considering that Jundia. (Rhamdia quelen) is a Neotropical catfish with great economical importance and useful experimental model, little information is available on basic aspects of its reproductive biology, especially on spermatogenesis. As a result, this study aimed to characterize the male germ cells, estimate the duration of spermatogenesis and evaluate the expression of selected stem cell genes in Jundia testis. Similar to other fish species, our results showed a remarkable decrease of germ cell nuclear volume during Jundia spermatogenesis, particularly from type A undifferentiated to late type B spermatogonia and from diplotene to late spermatids. Using a S-phase marker, bromodeoxyuridine (BrdU), the combined duration of meiotic and spermiogenic phases in this species was estimated in approximately 7 days. This is considered very short when compared to mammals, where spermatogenesis last from 30 to 74 days. Selected stem cell genes were partially sequenced and characterized in Jundia testis. Expression analysis showed higher plzf and pou5f3 mRNA levels in the cell fractions enriched by type A undifferentiated spermatogonia. These results were further confirmed by in situ hybridization that showed strong signal of plzf and pou5f3 mRNA in type A undifferentiated spermatogonia. Altogether, these information will expand our knowledge of the reproductive biology of this species, contributing to improve its production and management, and also for biotechnological applications, such as germ cell transplantation. (AU)

FAPESP's process: 14/07620-7 - Spermatogonial stem cell bank to preserve the genotype of endangered and valuable teleost species
Grantee:Rafael Henrique Nóbrega
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 12/00423-6 - Paracrine and molecular regulation of the spermatogonial stem cell niche in zebrafish (Danio rerio) and the use of common carp (Cyprinus carpio) as a model to delay spermatogonial differentiation during puberty
Grantee:Rafael Henrique Nóbrega
Support type: Regular Research Grants
FAPESP's process: 13/22778-3 - Spermatogonial stem cell bank to preserve the genotype of endangered and valuable teleost species
Grantee:Emanuel Ricardo Monteiro Martinez
Support type: Scholarships in Brazil - Post-Doctorate