Andrade, Marco A. R.
Simao, Ana M. S.
Millan, Jose Luis
Ramos, Ana P.
Total Authors: 7
 Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil
 Univ Fed Uberlandia, Rua Ruy Correa, BR-38702018 Patos De Minas - Brazil
 Sanford Burnham Prebys Med Discovery Inst, La Jolla, CA 92037 - USA
Total Affiliations: 3
Archives of Biochemistry and Biophysics;
MAR 15 2019.
Web of Science Citations:
Tissue-nonspecific alkaline phosphatase (TNAP) is a key enzyme in the biomineralization process as it produces phosphate from a number of phospho-substrates stimulating mineralization while it also inactivates inorganic pyrophosphate, a potent mineralization inhibitor. We have previously reported on the reconstitution of TNAP on Langmuir monolayers as well as proteoliposomes. In the present study, thin films composed of dimyristoylphosphatidic acid (DMPA) were deposited on titanium supports by the Langmuir-Blodgett (LB) technique, and we determined preservation of TNAP's phosphohydrolytic activity after incorporation into the LB films. Increased mineralization was observed after exposing the supports containing the DMPA:TNAP LB films to solutions of phospho-substrates, thus evidencing the role of TNAP on the growth of calcium phosphates after immobilization. These coatings deposited on metallic supports can be potentially applied as osteoconductive materials, aiming at the optimization of bone-substitutes integration in vivo. (AU)