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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?

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Andrade, Marco A. R. [1, 2] ; Derradi, Rafael [1] ; Simao, Ana M. S. [1] ; Millan, Jose Luis [3] ; Ramos, Ana P. [1] ; Ciancaglini, Pietro [1] ; Bolean, Mayte [1]
Total Authors: 7
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Fed Uberlandia, Rua Ruy Correa, BR-38702018 Patos De Minas - Brazil
[3] Sanford Burnham Prebys Med Discovery Inst, La Jolla, CA 92037 - USA
Total Affiliations: 3
Document type: Journal article
Source: Archives of Biochemistry and Biophysics; v. 663, p. 192-198, MAR 15 2019.
Web of Science Citations: 2

Tissue-nonspecific alkaline phosphatase (TNAP) is a key enzyme in the biomineralization process as it produces phosphate from a number of phospho-substrates stimulating mineralization while it also inactivates inorganic pyrophosphate, a potent mineralization inhibitor. We have previously reported on the reconstitution of TNAP on Langmuir monolayers as well as proteoliposomes. In the present study, thin films composed of dimyristoylphosphatidic acid (DMPA) were deposited on titanium supports by the Langmuir-Blodgett (LB) technique, and we determined preservation of TNAP's phosphohydrolytic activity after incorporation into the LB films. Increased mineralization was observed after exposing the supports containing the DMPA:TNAP LB films to solutions of phospho-substrates, thus evidencing the role of TNAP on the growth of calcium phosphates after immobilization. These coatings deposited on metallic supports can be potentially applied as osteoconductive materials, aiming at the optimization of bone-substitutes integration in vivo. (AU)

FAPESP's process: 17/08892-9 - Bioactive surfaces designed from Langmuir-Blodgett Films and Biominerals
Grantee:Ana Paula Ramos
Support type: Regular Research Grants
FAPESP's process: 14/11941-3 - Are collagen and Annexin V responsible for the control in the biomineralization process?
Grantee:Pietro Ciancaglini
Support type: Regular Research Grants
FAPESP's process: 16/21236-0 - Extracellular matrix vesicles (MVs) mimetic systems to study the regulation of the biomineralization process: proteoliposomes containing NPP1 and Annexin V
Grantee:Pietro Ciancaglini
Support type: Regular Research Grants
FAPESP's process: 15/00345-3 - Lipid microdomains in Langmuir-Blodgett films rich in alkaline phosphatase to obtain a Ti surface osteoinductive
Grantee:Marco Aurélio Raz de Andrade
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/20946-3 - Study of the growth of calcium phosphates and carbonates on organic matrices deposited on mettalic supports: obtainment of osteoinductive biomaterials
Grantee:Ana Paula Ramos
Support type: Regular Research Grants