Proteomic profile of root canal contents in teeth with post-treatment endodontic disease

Author(s)

Francisco, P. A. ^{[1, 2]} ; Delboni, M. G. ^{[2, 3]} ; Lima, A. R. ^[2] ; Xiao, Y. ^[1] ; Siqueira, W. L. ^[1] ; Gomes, B. P. F. A. ^[2]

Affiliation

^[1] Univ Western Ontario, Schulich Sch Med & Dent, Dept Biochem, London, ON - Canada

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Endodont Div, Dept Restorat Dent, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[3] DeVry Facid Univ, Coll Dent, Teresina, Piaui - Brazil

• Document type: Journal article
• Source: International Endodontic Journal; v. 52, n. 4, p. 451-460, APR 2019.
• Web of Science Citations: 0

Abstract

Aim To characterize the proteome of 20 root canals in teeth with post-treatment endodontic disease using mass spectrometry and to correlate the identified proteins with clinical features. Methodology Twenty patients with radiographic evidence of apical periodontitis and need for root canal re-treatment were selected. Samples from the root canal contents were collected and processed using two-dimensional capillary nano-flow liquid chromatography and electrospray ionization tandem mass spectrometry. The acquired spectra were separately searched against specific protein database. The results obtained were analysed using descriptive statistics. Additionally, Pearson's chi-square test or one-sided Fisher's exact test, as appropriate, was chosen to examine the null hypothesis that there is no relationship between each clinical feature and the presence of specific microbial or human proteins. Significance levels were set at 5% (P < 0.05). Results A total of 1153 human and 720 microbial UniProt accession numbers corresponding to proteins were recovered. The greater prevalence of proteins was related to biological functions, such as cellular and metabolic processes. A considerable number of microbial proteins with clinical relevance functions, such as pathogenesis/virulence, proteolysis, cell adhesion and drug resistance, were detected. Common endodontic pathogens related to post-treatment endodontic disease such as Enterococcus spp., Propionibacterium spp. and Streptococcus spp. were associated with 23, 40 and 94 distinct proteins, respectively. As for human proteins, many factors related to the immune system process were detected. No significant correlations were found between microbial and human proteins and the clinical features investigated (P > 0.05). Conclusions A considerable number of microbial and human proteins were identified using proteomic analyses, being mainly related to processes indicating cell viability. No significant correlation was found between proteins and clinical features. These findings suggest a network of important microbial pathogenic functions that may be responsible for the host immune system response. (AU)

• FAPESP's process: 17/16516-7 - Investigation of the microbiological content, matrix metalloproteinase expression and antimicrobial susceptibility of specific bacteria in endodontic failure cases
• Grantee: Priscila Amanda Francisco
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/23479-5 - Microbiomes and immunobiological aspects in endodontic infections
• Grantee: Brenda Paula Figueiredo de Almeida Gomes
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/19743-1 - Metaproteomics analyses of root canal content of teeth with endodontic failure
• Grantee: Priscila Amanda Francisco
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 15/19215-2 - Microorganisms and their virulence factors detection in saliva, pulp chamber and root canal of teeth associated with endodontic failure
• Grantee: Priscila Amanda Francisco
• Support Opportunities: Scholarships in Brazil - Master