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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Relationship between hypertension and periapical lesion: an in vitro and in vivo study

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Author(s):
Martins, Christine Men [1] ; Sasaki, Hajime [2] ; Hirai, Kimito [2] ; Andrada, Ana Cristina [3] ; Gomes-Filho, Joao Eduardo [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista Unesp, Aracatuba Dent Sch, Dept Restorat Dent, Aracatuba, SP - Brazil
[2] Forsyth Inst, Dept Immunol & Infect Dis, Cambridge, MA - USA
[3] Harvard Sch Dent Med, Div Endodont, Dept Restorat Dent & Biomat Sci, Boston, MA - USA
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Oral Research; v. 30, 2016.
Web of Science Citations: 0
Abstract

The aim of this study was to compare potential aspects of periapical lesion formation in hypertensive and normotensive conditions using hypertensive (BPH/2J) and wild-type control (BPN/3J) mice. The mandibular first molars of both strains had their dental pulp exposed. At day 21 the mice were euthanized and right mandibular molars were used to evaluate the size and phenotype of apical periodontitis by microCT. Proteins were extracted from periapical lesion on the left side and the expressions of IL1 alpha, IL1 beta and TNF alpha were analyzed by ELISA. Bone marrow stem cells were isolated from adult mice femurs from 2 strains and osteoclast differentiation was evaluated by tartrate-resistant acid phosphatase (TRAP) in vitro. The amount of differentiated osteoclastic cells was nearly double in hypertensive mice when compared to the normotensive strain (p < 0.03). Periapical lesion size did not differ between hypertensive and normotensive strains (p > 0.7). IL1 alpha, IL1 beta and TNF alpha cytokines expressions were similar for both systemic conditions (p > 0.05). Despite the fact that no differences could be observed in periapical lesion size and cytokines expressions on the systemic conditions tested, hypertension showed an elevated number of osteoclast differentiation. (AU)

FAPESP's process: 13/09446-1 - Influence of hypertension in tissue response and mineralization capacity of cement repairers MTA Angelus® white and grey and IRM®. Histology and immunohistochemistry analysis in rats.
Grantee:Christine Men Martins
Support Opportunities: Scholarships in Brazil - Doctorate