| Full text | |
| Author(s): |
Bombonato, Juliana Rodrigues
[1, 2]
;
Silva Bonatelli, Isabel Aparecida
[1]
;
Rodrigues Silva, Gislaine Angelica
[1]
;
Moraes, Evandro Marsola
[1]
;
Zappi, Daniela Cristina
[3]
;
Taylor, Nigel P.
[4]
;
Franco, Fernando Faria
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Carlos, Ctr Ciencias Humanas & Biol, Dept Biol, Rodovia Joao Leme Santos, SP264 Km 110, BR-18052780 Sorocaba, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Programa Posgrad Biol Comparada, Ribeirao Preto, SP - Brazil
[3] Museu Paraense Emilio Goeldi, Inst Tecnol Vale, Coord Bot, Belem, Para - Brazil
[4] Natl Pk Board, Singapore Bot Gardens, Singapore - Singapore
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | GENETICS AND MOLECULAR BIOLOGY; v. 42, n. 1, p. 87-94, JAN-MAR 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Abstract The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus. (AU) | |
| FAPESP's process: | 14/25227-0 - Multilocus phylogenetic analysis to recover evolutionary relationships among species of genus Cereus (Cactaceae; Cereeae) |
| Grantee: | Fernando de Faria Franco |
| Support type: | Regular Research Grants |