| Full text | |
| Author(s): |
Ribeiro Fernandes, Juliane Cristina
[1, 2]
;
Aoki, Juliana Ide
[1]
;
Acuna, Stephanie Maia
[1]
;
Zampieri, Ricardo Andrade
[1]
;
Markus, Regina P.
[1]
;
Floeter-Winter, Lucile Maria
[1]
;
Muxel, Sandra Marcia
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Med Trop, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 9, MAR 20 2019. |
| Web of Science Citations: | 2 |
| Abstract | |
Leishmaniases are neglected diseases that cause a large spectrum of clinical manifestations, from cutaneous to visceral lesions. The initial steps of the inflammatory response involve the phagocytosis of Leishmania and the parasite replication inside the macrophage phagolysosome. Melatonin, the darkness-signaling hormone, is involved in modulation of macrophage activation during infectious diseases, controlling the inflammatory response against parasites. In this work, we showed that exogenous melatonin treatment of BALB/c macrophages reduced Leishmania amazonensis infection and modulated host microRNA (miRNA) expression profile, as well as cytokine production such as IL-6, MCP-1/CCL2, and, RANTES/CCL9. The role of one of the regulated miRNA (miR-294-3p) in L. amazonensis BALB/c infection was confirmed with miRNA inhibition assays, which led to increased expression levels of Tnf and Mcp-1/Ccl2 and diminished infectivity. Additionally, melatonin treatment or miR-30e-5p and miR-302d-3p inhibition increased nitric oxide synthase 2 (Nos2) mRNA expression levels and nitric oxide (NO) production, altering the macrophage activation state and reducing infection. Altogether, these data demonstrated the impact of melatonin treatment on the miRNA profile of BALB/c macrophage infected with L. amazonensis defining the infection outcome. (AU) | |
| FAPESP's process: | 16/19815-2 - Role of miRNAs in the TLRs-mediated immune response to Leishmania amazonensis infection |
| Grantee: | Sandra Marcia Muxel |
| Support type: | Regular Research Grants |
| FAPESP's process: | 12/15263-4 - Alterations in the composition of microRNAs in macrophages infected by Leishmania amazonensis |
| Grantee: | Sandra Marcia Muxel |
| Support type: | Regular Research Grants |
| FAPESP's process: | 14/50717-1 - Biochemical, physiological and functional genomic studies of Leishmania-macrophage interaction |
| Grantee: | Lucile Maria Floeter-Winter |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/23519-2 - Analysis of role of miRNAs and transcription factors on the regulation of gene expression of Leishmania amazonensis infected murine macrophages |
| Grantee: | Stephanie Maia Acuna |
| Support type: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 16/03273-6 - Dual transcriptome profiling of murine macrophages infected with Leishmania amazonensis |
| Grantee: | Juliana Ide Aoki |
| Support type: | Scholarships in Brazil - Post-Doctorate |