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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A plant proteinase inhibitor from Enterolobium contortisiliquum attenuates airway hyperresponsiveness, inflammation and remodeling in a mouse model of asthma

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Dias Rodrigues, Adriana Palmeira [1] ; Santos Bortolozzo, Anelize Sartori [1] ; Arantes-Costa, Fernanda Magalhaes [1] ; Saraiva-Romanholo, Beatriz Mangueira [2, 1] ; Ribas de Souza, Flavia Castro [1] ; Bruggemanni, Thayse Regina [1] ; Roncon Santana, Fernanda Paula [1] ; de Brito, Marlon Vilela [3] ; Bonturi, Camila Ramalho [3] ; dos Santos Nunes, Natalia Neto [3] ; Prado, Carla Maximo [4] ; Leick, Edna Aparecida [1] ; Vilela Oliva, Maria Luiza [3] ; Martins, Milton de Arruda [1] ; Righetti, Renato Fraga [1, 5] ; Lopes Calvo Tiberio, Iolanda de Fatima [1]
Total Authors: 16
[1] Univ Sao Paulo, Fac Med FMUSP, Dept Clin Med, Sao Paulo - Brazil
[2] Univ City Sao Paulo UNICID, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Dept Biosci, Santos - Brazil
[5] Hosp Sirio Libanes, Dept Reabilitacao, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: HISTOLOGY AND HISTOPATHOLOGY; v. 34, n. 5, p. 537-552, MAY 2019.
Web of Science Citations: 0

Introduction. Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for asthma. Purpose. The aim of the present study was to evaluate the effects of Enterolobium contortisiliquum trypsin inhibitor (EcTI) on pulmonary mechanical function, eosinophilic recruitment, inflammatory cytokines, remodeling and oxidative stress in an experimental model of chronic allergic pulmonary inflammation. Methods. BALB/c mice were divided into 4 groups: C (saline i.p and inhalations with saline), OVA (ovalbumin i.p and inhalations with ovalbumin); C+EC (saline i.p, inhalations with s aline and treatment with EcTI); OVA+EC (ovalbumin i.p, inhalations with ovalbumin and treatment with EcTI). On day 29, we performed the following tests: resistance (Rrs) and elastance (Ers) of the respiratory system; (b) quantify eosinophils, 8-ISO-PGF2 alpha, collagen and elastic fiber volume fractions; (c) IFN-gamma, IL-4, IL-5, IL-13, MMP-9, TIMP-1,TGF-beta, iNOS and p65-NF kappa B-positive cells in the airway and alveolar walls. Results. In OVA+EC group, there was an attenuation of the Rrs and Ers, reduction of eosinophils, IL-4, IL-5, IL-13, IFN-gamma, iNOS and p65-NF kappa B-positive cells compared to OVA group. The 8-ISO-PGF2 alpha, elastic and collagen fibers volume fractions as well as the positive cells for MMP-9, TIMP-1 and TGF-beta positive cells were decreased in OVA+EC compared to the OVA group. Conclusion. EcTI attenuates bronchial hyperresponsiveness, inflammation, remodeling and oxidative stress activation in this experimental mouse model of asthma. (AU)

FAPESP's process: 13/17944-1 - Anti-IL-17 in the modulation of lung mechanics, inflammation, oxidative stress, extracellular matrix remodeling in mice with chronic pulmonary inflammation associated or not with acute lung injury induced by LPS acute lung injury by LPS
Grantee:Iolanda de Fátima Lopes Calvo Tibério
Support type: Regular Research Grants