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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Extracellular L-asparaginase production in solid-state fermentation by using sugarcane bagasse as support material

Full text
Author(s):
Muso Cachumba, J. J. [1] ; Teran Hilares, R. [1] ; Brumano, L. P. [1] ; Marcelino, P. R. F. [1] ; Antunes, F. A. F. [1] ; Santos, J. C. [1] ; da Silva, S. S. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Engn Sch Lorena, Dept Biotechnol, BR-12602810 Lorena, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY; v. 49, n. 4, p. 328-333, APR 21 2019.
Web of Science Citations: 0
Abstract

L-asparaginase is an important enzyme used in the pharmaceutical and food industry, which can be produced by different microorganisms using low cost feedstocks. In this work, sugarcane bagasse (SCB) was used as support for enzyme production in solid-state fermentation (SSF) by A. terreus. Initially, the influence of the variables carbon and nitrogen sources on the enzyme production was studied following an experimental design carried out in Erlenmeyer flasks. Statistical analysis indicated the use of 0.54% of starch, 0% of maltose, 0.44% of asparagine, and 1.14% of glutamine in the medium, resulting in enzyme activity per volume of produced extract of 120.723 U/L. Then, these conditions were applied in a horizontal column reactor filled with SCB, producing 105.3 U/L of enzyme activity. Therefore, the potential of extracellular L-asparaginase enzyme production in the column reactor using sugarcane bagasse as support was demonstrated and it represents a system that can favor large scale production. (AU)

FAPESP's process: 14/27055-2 - Processes intensification of lignocellulosic biorefineries integrating ethanol and xylitol production steps from sugarcane bagasse in fluidized bed bioreactor
Grantee:Felipe Antonio Fernandes Antunes
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 17/11086-4 - Development of innovative approaches for the main stages of 2G ethanol production: continuous pretreatment using hydrodynamic cavitation and evaluation of SSCF process with interconnected reactors
Grantee:Ruly Teran Hilares
Support Opportunities: Scholarships in Brazil - Post-Doctoral