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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protein PEGylation for the design of biobetters: from reaction to purification processes

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Author(s):
Picado Madalena Santos, Joao Henrique [1, 2] ; Torres-Obreque, Karin Mariana [1] ; Meneguetti, Giovanna Pastore [1] ; Amaro, Beatriz Panichi [1] ; Rangel-Yagui, Carlota Oliveira [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem Pharmaceut Technol, Sao Paulo - Brazil
[2] Univ Aveiro, Aveiro Inst Mat, Dept Chem, CICECO, Aveiro - Portugal
Total Affiliations: 2
Document type: Review article
Source: Brazilian Journal of Pharmaceutical Sciences; v. 54, n. SI 2018.
Web of Science Citations: 7
Abstract

The covalent attachment of polyethylene glycol (PEG) to therapeutical proteins is an important route to develop biobetters for biomedical, biotech and pharmaceutical industries. PEG conjugation can shield antigenic epitopes of the protein, reduce degradation by proteolytic enzymes, enhance long-term stability and maintain or even improve pharmacokinetic and pharmacodynamics characteristics of the protein drug. Nonetheless, correct information in terms of the PEGylation process from reaction to downstream processing is of paramount importance for the industrial application and processing scale-up. In this review we present and discuss the main steps in protein PEGylation, namely: PEGylation reaction, separation of the products and final characterization of structure and activity of the resulting species. These steps are not trivial tasks, reason why bioprocessing operations based on PEGylated proteins relies on the use of analytical tools according to the specific pharmaceutical conjugate that is being developed. Therefore, the appropriate selection of the technical and analytical methods may ensure success in implementing a feasible industrial process. (AU)

FAPESP's process: 16/22065-5 - N-terminal pegylation of proteins and purification by aqueous two-phase systems
Grantee:Carlota de Oliveira Rangel Yagui
Support type: Regular Research Grants