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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dehydration as a stressor in toads (Rhinella ornata)

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Author(s):
Giorgi Barsotti, Adriana Maria [1] ; Titon Junior, Braz [1] ; Monteiro Titon, Stefanny Christie [1] ; Gomes, Fernando Ribeiro [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY; v. 331, n. 3, p. 168-174, MAR 2019.
Web of Science Citations: 0
Abstract

Availability of a humid environment is essential for amphibians to carry out their activities and most species are characterized by low resistance to evaporative water loss. Moreover, dehydration severely compromises amphibian locomotor and foraging performance, representing a relevant selective factor modulating the evolution of its integrative phenotype. In this way, we hypothesized that dehydration is a stressor for toads, inducing a stress response comparable to that elicited by another commonly used stress protocol: restraint challenge. We evaluated changes in plasma levels of corticosterone (CORT), hematocrit (Hct), and neutrophil:lymphocyte (N:L) ratio in adult males of Rhinella ornata, experimentally submitted to different levels of hydration (100%, 90%, and 80% of standard body mass) and to restraint challenge. Our results showed that dehydrating toads by 10% increase CORT to levels equivalent to that obtained by restraint. Moreover, toads dehydrated by 20% show a more pronounced increase in CORT, along with increased Hct and N:L ratio. In this way, we corroborated the hypothesis that dehydration triggers a pronounced stress response in R. ornata. Highlights Dehydration can elicit a stress response in toads, by increasing corticosterone plasma levels and neutrophil:lymphocyte ratio. Corticosterone plasma levels were higher in individuals 20% dehydrated than in subjects submitted to restriction of movement, showing that dehydration may offer high intensity stress. (AU)

FAPESP's process: 13/00900-1 - Comparative study of the relationships between immunocompetence and plasmatic levels of gonadal and inter-renal steroids in toads
Grantee:Fernando Ribeiro Gomes
Support type: Regular Research Grants
FAPESP's process: 16/05024-3 - Hidric stress and immune response in frogs (Rhinella ornata) inhabiting Atlantic Forest fragments
Grantee:Adriana Maria Giorgi Barsotti
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/16320-7 - Impacts of climate/environmental change on the fauna: an integrative approach
Grantee:Carlos Arturo Navas Iannini
Support type: Research Program on Global Climate Change - Thematic Grants