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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens

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Author(s):
Stan, Razvan C. [1] ; Francoso, Katia S. [2] ; Alves, Rubens P. S. [3] ; Ferreira, Luis Carlos S. [3] ; Soares, Irene S. [2] ; de Camargo, Maristela M. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Dept Immunol, Inst Biomed Sci, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Clin & Toxicol Anal, Sch Pharmaceut Sci, Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Microbiol, Inst Biomed Sci, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS Neglected Tropical Diseases; v. 13, n. 4 APR 2019.
Web of Science Citations: 0
Abstract

Fever is a regulated increase of the body temperature resulting from both infectious and non-infectious causes. Fever is known to play a role in modulating immune responses to infection, but the potential of febrile temperatures in regulating antigen binding affinity to antibodies has not been explored. Here we investigated this process under in vitro conditions using Isothermal titration calorimetry and ELISA. We used selected malarial and dengue antigens against specific monoclonal antibodies, and observed a marked increase in the affinity of these antibody-antigen complexes at 40 degrees C, compared to physiological (37 degrees C) or pathophysiological temperatures (42 degrees C). Induced thermal equilibration of the protein partners at these temperatures in vitro, prior to measurements, further increased their binding affinity. These results suggest another positive and adaptive role for fever in vivo, and highlight the favourable role of thermal priming in enhancing protein-protein affinity for samples with limited availability. (AU)

FAPESP's process: 11/51778-6 - Functional study of the Unfolded Protein Response in human b lymphocytes: primary hypogammaglobulinemia as a model for dysfunctional UPR
Grantee:Maristela Martins de Camargo
Support type: Regular Research Grants
FAPESP's process: 12/13032-5 - Generation and analysis of the immunogenicity of recombinant proteins based on the different allelic forms of the circumsporozoite antigen of Plasmodium vivax aiming at the development of a universal vaccine against malaria
Grantee:Irene da Silva Soares
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/17595-0 - New vaccine research based on recombinant proteins of dengue virus
Grantee:Luis Carlos de Souza Ferreira
Support type: Regular Research Grants
FAPESP's process: 16/20045-7 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV)
Grantee:Luis Carlos de Souza Ferreira
Support type: Research Projects - Thematic Grants