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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Controlling parameters and characteristics of electrochemical biosensors for enhanced detection of 8-hydroxy-2 `-deoxyguanosine

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Author(s):
Faria, Aline M. [1] ; Peixoto, Elisa B. M. I. [1] ; Adamo, Cristiane B. [1] ; Flacker, Alexandre [1] ; Longo, Elson [2] ; Mazon, Talita [1]
Total Authors: 6
Affiliation:
[1] CTI, Ctr Tecnol Informacao Renato Archer, Rod D Pedro I, KM 143-6, BR-13069901 Campinas, SP - Brazil
[2] Univ Fed Sao Carlos, CDMF, POB 676, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, MAY 15 2019.
Web of Science Citations: 0
Abstract

This work discusses the parameters and characteristics required on the development of a scalable and reliable electrochemical sensor board for detecting 8-hydroxy-2'-deoxyguanosine (8-OHdG), an oxidative stress biomarker for diabetic nephropathy, cancer and Parkinson's disease. We used Printed Circuit Board (PCB) technology to make a precise, low-cost bare sensor board. ZnO nanorods (NRs) and ZnO NRs: reduced graphene oxide (RGO) composites were used as a pathway for antibody immobilization on the working electrode (WE). The parameters and characteristics of the WE were controlled for enhancing the quality of the electrochemical sensor board. Thickness of the gold and the presence of ZnO NRs or their composite on the WE have influence on charge transference process and reproducibility of the sensor board. The amount of the antibody, and its incubation period are crucial to avoid saturation of the sites during immobilization step and reduce the cost of the sensor. Our ZnO NRs-based electrochemical sensor board showed high sensitivity and selectivity to 8-OHdG with detection capacity in the range of 0.001-5.00 ng. mL(-1). The successful application of our immunosensor to detect 8-OHdG in urine was evidenced. (AU)

FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
Grantee:Elson Longo da Silva
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC