Genetic diversity of Anaplasma marginale in beef cattle in the Brazilian Pantanal

Author(s):Show less -

de Souza Ramos, Inalda Angelica ^{[1, 2]} ; Herrera, Heitor Miraglia ^[3] ; Fernandes, Simone de Jesus ^[1] ; do Amaral, Renan Bressianini ^[1] ; de Souza Zanatto, Diego Carlos ^[1] ; Viera da Silva, Thiago Merighi ^[1] ; Horta, Bruna le Senechal ^[1] ; Vilela Campos, Joao Bosco ^[3] ; Almeida Alves, Joao Vitor ^[3] ; de Macedo, Gabriel Carvalho ^[3] ; Machado, Rosangela Zacarias ^[1] ; Andre, Marcos Rogerio ^[1]

Affiliation

^[1] Univ Estadual Paulista, Unesp, Fac Agrarian & Vet Sci, Jaboticabal, SP - Brazil

^[2] State Univ Stio Paulo UNESP, Fac Agrarian & Vet Sci, UNESP Jaboticabar, Grad Program Vet Med, Anim Pathol, Hostparasite Rel, Jaboticabal, SP - Brazil

^[3] Don Bosco Catholic Univ UCDB, Campo Grande, MS - Brazil

• Document type: Journal article
• Source: TICKS AND TICK-BORNE DISEASES; v. 10, n. 4, p. 805-814, JUN 2019.
• Web of Science Citations: 1

Abstract

There are few studies on the genetic diversity of Anaplasma marginale in Brazilian cattle herds, especially about beef cattle. The objective of this study was to evaluate the genetic diversity of A. marginale, based on the msp1 alpha gene in Bos taurus indicus sampled from the Brazilian Pantanal. Aliquots of blood with and without EDTA were taken from 400 cattle (200 cows and 200 calves) across five extensive farms. The samples were submitted to the indirect immunoenzymatic assay (iELISA), quantitative real-time PCR (qPCR) for the msp1 beta gene and to the semi-nested (sn) PCR for the msp1 alpha gene. Positive samples were sequenced by the Sanger method and subjected to diversity analysis using the RepeatAnalyser software. The percentage of positive animals by iELISA, qPCR and (sn) PCR was 72.2% (289/400), 56.7% (227/400) and 23% (52/227), respectively. Cows (154/200) showed to be significantly more seropositive than calves (135/200). In qPCR, the number of calves and average quantification value (138/200; 1.3 x 10(6)) A. marginale msp1 alpha copies per mu L proved to be higher when compared to that found for the cows (89/200; 3.9 x 10(4)). The microsatellite analysis of the 26 sequences obtained from the msp1 alpha gene revealed the presence of E (77%), C (15.4%) and B (7.7%) genotypes. Fourteen A. marginale strains were identified in the studied region, with eight that have never before been described in the literature (tau-10-13 - 13-18; tau-27-18; EV8-EV8-17; alpha-beta-beta-beta- 100; EV7-11 -10-15; tau-11-11 - 27-18; tau-11-10-15; tau-27-13-18). Beef cattle are highly exposed to A. marginale in the Brazilian Pantanal. Moreover, a high genetic diversity of A. marginale, with eight new strains, was found in the studied region. While cows may act as chronic carriers, perpetuating the pathogen within the herd, male beef calves sold to other regions may disperse these strains. (AU)

• FAPESP's process: 15/14896-1 - Molecular detection and characterization of agents belonging to Anaplasmataceae e Bartonellaceae family in wild animals and ectoparasites in Brazil
• Grantee: Marcos Rogério André
• Support Opportunities: Regular Research Grants