Microwires of Au-Ag Nanocages Patterned via Magnetic Nanoadhesives for Investigating Proteins using Surface Enhanced Infrared Absorption Spectroscopy

Author(s)

Melo, Antonio F. A. A. ^{[1, 2, 3]} ; Hassan, Ayaz ^[1] ; Macedo, Lucyano J. A. ^[1] ; Osica, Izabela ^{[2, 4]} ; Shrestha, Lok Kumar ^[2] ; Ji, Qingmin ^{[2, 5]} ; Oliveira, Jr., Osvaldo N. ^[6] ; Henzie, Joel ^[2] ; Ariga, Katsuhiko ^{[2, 7]} ; Crespilho, Frank N. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Chem, BR-13560970 Sao Carlos, SP - Brazil

^[2] NIMS, Res Ctr Mat Nanoarchitecton MANA, WPI, 1-1 Namiki, Tsukuba, Ibaraki 3050044 - Japan

^[3] Fed Inst Educ Sci & Technol Piaui, BR-64000040 Teresina, PI - Brazil

^[4] Warsaw Univ Technol, Fac Mat Sci & Engn, Woloska 141, PL-02507 Warsaw - Poland

^[5] Nanjing Univ Sci & Technol, Herbert Gleiter Inst Nanosci, 200 Xiaolingwei, Nanjing 210094, Jiangsu - Peoples R China

^[6] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Carlos, SP - Brazil

^[7] Univ Tokyo, Grad Sch Frontier Sci, Dept Adv Mat Sci, 5-1-5 Kashiwanoha, Kashiwa, Chiba 2778561 - Japan

• Document type: Journal article
• Source: ACS APPLIED MATERIALS & INTERFACES; v. 11, n. 20, p. 18053-18061, MAY 22 2019.
• Web of Science Citations: 3

Abstract

The controlled assembly of metal nanoparticles into ordered structures interacting with biological molecules is an emerging concept in surface science. Here, bare magnetite nanoparticles (Fe3O4-NPs) were employed as nanoadhesives to capture hollow metallic nanostructures (Au-Ag nanocages) from aqueous suspensions, and these coupled nanostructures were patterned onto various types of substrate via magneto-lithography. Microwires of Au-Ag nanocages patterned onto an Au subitrate behaved as optical antennas, providing a plasmonic enhancement exploited in surface-enhanced infrared absorption spectroscopy (SEIRAS) to investigate the proteins cytochrome c, bilirubin oxidase, alcohol dehydrogenase, bovine serum albumin, and glucose oxidase. Chemical maps containing more than 4000 spectra, acquired within only 2 min with a focal plane array detector, indicate that proteins were adsorbed along the microwires with their secondary structure preserved according to the spatial distribution of their amide groups. We believe there are significant practical aspects of the methodology proposed here to develop an alternative label-free assay for investigating biological molecules. (AU)

• FAPESP's process: 17/20493-2 - Study of metalloenzymes through electrochemistry coupled to vibrational spectroscopy
• Grantee: Lucyano Jefferson Alves de Macêdo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/25806-6 - Interaction between biomolecules and nanostructures: electrochemistry, interfaces and surfaces
• Grantee: Ayaz Hassan
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/04663-4 - Enzymatic bioelectrodes applied in biofuel cells: experimental and simulation
• Grantee: Frank Nelson Crespilho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/01541-0 - Development of bioanodes composed by glucose oxidase and fructose dehydrogenase and its recombinant structures for aplication in prototype of biofuel cell: power generation from biomass (sugar cane)
• Grantee: Frank Nelson Crespilho
• Support Opportunities: Regular Research Grants