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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Thermostable Aspergillus fumigatus GH7 Endoglucanase Over-Expressed in Pichia pastoris Stimulates Lignocellulosic Biomass Hydrolysis

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Author(s):
Bernardi, Aline Vianna [1] ; Yonamine, Deborah Kimie [1] ; Uyemura, Sergio Akira [2] ; Dinamarco, Taisa Magnani [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Chem Dept, Fac Philosophy Sci & Literature Ribeirao Preto, BR-14040901 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Dept Clin Toxicol & Bromatol Anal, Fac Pharmaceut Sci, BR-14040903 Ribeirao Preto - Brazil
Total Affiliations: 2
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 9 MAY 1 2019.
Web of Science Citations: 0
Abstract

In the context of avoiding the use of non-renewable energy sources, employing lignocellulosic biomass for ethanol production remains a challenge. Cellulases play an important role in this scenario: they are some of the most important industrial enzymes that can hydrolyze lignocellulose. This study aims to improve on the characterization of a thermostable Aspergillus fumigatus endo-1,4--glucanase GH7 (Af-EGL7). To this end, Af-EGL7 was successfully expressed in Pichia pastoris X-33. The kinetic parameters K-m and V-max were estimated and suggested a robust enzyme. The recombinant protein was highly stable within an extreme pH range (3.0-8.0) and was highly thermostable at 55 degrees C for 72 h. Low Cu2+ concentrations (0.1-1.0 mM) stimulated Af-EGL7 activity up to 117%. Af-EGL7 was tolerant to inhibition by products, such as glucose and cellobiose. Glucose at 50 mM did not inhibit Af-EGL7 activity, whereas 50 mM cellobiose inhibited Af-EGL7 activity by just 35%. Additionally, the Celluclast((R)) 1.5L cocktail supplemented with Af-EGL7 provided improved hydrolysis of sugarcane bagasse in natura, sugarcane exploded bagasse (SEB), corncob, rice straw, and bean straw. In conclusion, the novel characterization of Af-EGL7 conducted in this study highlights the extraordinary properties that make Af-EGL7 a promising candidate for industrial applications. (AU)

FAPESP's process: 18/11231-7 - Functional characterization of GH7 endoglucanase from Aspergillus fumigatus
Grantee:Deborah Kimie Yonamine
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/10466-0 - Transcriptome analysis of Aspergillus fumigatus grown on sugarcane bagasse
Grantee:Taisa Magnani Dinamarco
Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants