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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Reduced intestinal FADS1 gene expression and plasma omega-3 fatty acids following Roux-en-Y gastric bypass

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Garla, Priscila [1] ; Sala, Priscila [1] ; Matos Torrinhas, Raquel Susana [1] ; Machado, Natasha Mendonca [1] ; Fonseca, Danielle Cristina [1] ; da Silva, Mariane Marques [1] ; Ravacci, Graziela Rosa [1] ; Belarmino, Giliane [1] ; Ishida, Robson Kiyoshi [1, 2] ; Mota Siqueira Guarda, Ismael Francisco [1] ; Hourneaux de Moura, Eduardo Guimardes [1, 2] ; Sakai, Paulo [1, 2] ; Santo, Marco Aurelio [1, 2] ; Cotrim Guerreiro da Silva, Ismael Dale [3] ; Alves Pereira, Claudia Cristina [3] ; Heymsfield, Steven [4] ; Cardillo Correa-Giannella, Maria Lucia [5, 6] ; Calder, Philip C. [7, 8, 9] ; Waitzberg, Dan Linetzky [1, 2]
Total Authors: 19
[1] Univ Sao Paulo, Fac Med FMUSP, Lab Metanutri LIM 35, Dept Gastroenterol, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo, Sao Paulo, SP - Brazil
[4] Pennington Biomed Res Ctr, 6400 Perkins Rd, Baton Rouge, LA 70808 - USA
[5] Univ Nove Julho, Programa posgrad Med, Sao Paulo - Brazil
[6] Univ Sao Paulo, Fac Med FMUSP, Lab Carboidrato & Radioimunoensaio LIM 18, Sao Paulo, SP - Brazil
[7] Univ Southampton, Southampton SO9 5NH, Hants - England
[8] Univ Southampton, Fac Med, Southampton SO9 5NH, Hants - England
[9] Univ Hosp Southampton NHS Fdn Trust, Southampton Biomed Res Ctr, Natl Inst Hlth Res, Southampton SO9 5NH, Hants - England
Total Affiliations: 9
Document type: Journal article
Source: Clinical Nutrition; v. 38, n. 3, p. 1280-1288, JUN 2019.
Web of Science Citations: 0

Background \& aims: Roux-en-Y gastric bypass (RYGB) limits food ingestion and may alter the intestinal expression of genes involved in the endogenous synthesis of polyunsaturated fatty acids (PUFAs). These changes may decrease the systemic availability of bioactive PUFA5 after RYGB. To study the impact of RYGB on the dietary ingestion and plasma concentration of PUFA5 and on the intestinal expression of genes involved in their endogenous biosynthesis in severely obese women with type 2 diabetes. Methods: Before, and 3 and 12 months after RYGB, obese women (n = 20) self-reported a seven-day dietary record, answered a food frequency query and provided plasma samples for alpha-linolenic (ALA), eicosapentaenoic (EPA), docosahexaenoic (DHA) and arachidonic (ARA) acid assessment by gas chromatography. Intestinal biopsies (duodenum, jejunum and ileum) were collected through double balloon endoscopy before and 3 months after RYGB for gene expression analysis by microarray (Human GeneChip 1.0 ST array) and RT-qPCR validation. Results: Compared to the preoperative period, patients had decreased intakes of PUFAs, fish and soybean oil (p < 0.05) and lower plasma concentrations of ALA and EPA (p < 0.001) 3 and 12 months after RYGB. FADS] gene expression was lower in duodenum (RT-qPCR fold change = 1.620, p < 0.05) and jejunum (RT-qPCR fold change = -1.549, p < 0.05) 3 months following RYGB, compared to before surgery. Conclusion: RYGB decreased PUFA ingestion, plasma ALA and EPA levels, and intestinal expression of FADS] gene. The latter encodes a key enzyme involved in endogenous biosynthesis of PUFA5. These data suggest that supplementation of omega-3 PUFAs may be required for obese patients undergoing RYGB. Clinical Trial Registry number and website: NCT01251016; Plataforma Brasil - 19339913.0.0000.0068. (C) 02018 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. (AU)

FAPESP's process: 11/09612-3 - Changes in gene expression in gastric and intestinal tissue of type 2 Diabetes patients submitted to Roux-en-Y gastric bypass
Grantee:Dan Linetzky Waitzberg
Support type: Research Projects - Thematic Grants