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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Parvovirus B19 seroprevalence, viral load, and genotype characterization in volunteer blood donors from southern Brazil

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Author(s):
Slavov, Svetoslav N. [1, 2] ; Rodrigues, Evandra S. [2] ; Sauvage, Virginie [3] ; Caro, Valerie [4] ; Diefenbach, Cristiane F. [5] ; Zimmermann, Ana M. [6] ; Covas, Dimas T. [2] ; Laperche, Syria [3] ; Kashima, Simone [2]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Internal Med, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Blood Ctr Ribeirao Preto, Lab Mol Biol, Rua Tenente Catao Roxo 2501, BR-14051140 Ribeirao Preto, SP - Brazil
[3] INTS, Dept Etud Agents Transmissibles Sang DATS, Ctr Natl Reference Risques Infect Transfus, Paris - France
[4] Inst Pasteur, PGP, Lab Urgent Response Biol Threats, Environm & Infect Risks Res & Expertise Unit, Paris - France
[5] Univ Fed Santa Maria, Univ Hosp Santa Maria, Dept Internal Med, Santa Maria, RS - Brazil
[6] Hosp Dr Astrogildo de Azevedo, Hemotherapy Serv, Hematol Dept, Santa Maria, RS - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Journal of Medical Virology; v. 91, n. 7, p. 1224-1231, JUL 2019.
Web of Science Citations: 0
Abstract

Usually transmitted via respiratory droplets, parvovirus B19 (B19V) can also be acquired by blood transfusion especially because of viral persistence, resistance to blood treatment procedures, and high viral load during the early infection phase. This is particularly problematic in immunocompromised or anemic patients where the infection can have a severe outcome. As B19V DNA was detected in blood donations from South Brazil during a viral metagenomic survey performed by Next-Generation Sequencing, the objective of this retrospective study was to evaluate the seroprevalence, B19V DNA presence and circulating genotypes in a Hospital Blood Transfusion Service in Santa Maria city in South Brazil (Rio Grande do Sul state). Among 480 volunteer blood donors, 53.9% (n = 258 of 479) were anti-B19V IgG-positive, and 9 (1.9%) plasma samples presented B19V DNA. In almost all cases (n = 7 of 9, 77.8%), B19V DNA load was accompanied by the presence of anti-B19V IgG suggesting a persistent infection. The sequencing of the strains demonstrated that all belong to genotype 1 which is the most prevalent worldwide. The analysis of the recipient information of the positive for B19V DNA units revealed no related posttransfusion adverse effects. Our results demonstrate for the first time, B19V seroprevalence, viral load, and genotypes among blood donors from South Brazil and give a light for the circulation and impact of this B19V in this part of the country. (AU)

FAPESP's process: 09/16623-1 - Molecular pathogenesis and clinical consequences of the infections caused by the human parvovirus B19 and Human Cytomegalovirus among patients with hemaglobinopathies and haemophilia
Grantee:Svetoslav Nanev Slavov
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/23205-8 - Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem cell transplantation by multiple-research approach
Grantee:Svetoslav Nanev Slavov
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 14/50947-7 - INCT 2014: in Stem Cell and Cell Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Projects - Thematic Grants