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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells

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Author(s):
Rovai, Emanuel Silva [1] ; Batitucci Ambrosio, Lucas Macedo [1] ; de Franca, Bruno Nunes [1] ; de Oliveira, Leticia Rodrigues [1] ; Gasparoni, Leticia Miquelitto [1] ; Sipert, Carla Renata [2] ; Holzhausen, Marinella [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Div Periodont, Dept Stomatol, Sch Dent, Sao Paulo - Brazil
[2] Univ Sao Paulo, Div Endodont, Dept Restorat Dent, Sch Dent, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: STEM CELLS INTERNATIONAL; 2019.
Web of Science Citations: 0
Abstract

Protease-activated receptor 1 (PAR(1)) has been associated to tissue repair and bone healing. The aim of the present study was to evaluate the effect of PAR(1) activation on the osteogenic activity of human periodontal ligament stem cells (PDLSCs). PDLSCs were cultured in the presence of PAR(1)-selective agonist peptide (100nM), thrombin (0.1U/mL), or PAR(1) antagonist peptide (100nM). Calcium deposits, calcium concentration (supernatant), alkaline phosphatase activity (ALP), cell proliferation, and gene (qPCR) and protein expression (ELISA assay) of osteogenic factors were assessed at 2, 7, and 14 days. PAR(1) activation led to increased calcium deposits (p<0.05), calcium concentration (p<0.05), ALP activity (p<0.05), and cell proliferation (p<0.05). Further, PAR(1) activation may increase gene and protein expression of Runx2 (p<0.05) and OPG (p<0.05). In conclusion, PAR(1) activation increases osteogenic activity of PDLSCs, providing a possible new strategy for periodontal regenerative therapies. (AU)

FAPESP's process: 15/23746-3 - Protease activated receptor 1 (PAR1) activation effect on osteogenic cells od the periodontal ligament
Grantee:Letícia Rodrigues de Oliveira
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/07396-2 - Effect of protease activated receptor 1 (PAR1) activation on osteogenic activity of periodontal ligament cells
Grantee:Marinella Holzhausen Caldeira
Support type: Regular Research Grants
FAPESP's process: 16/11399-0 - Effect of activation of Protease-Activated Receptor type 2 (PAR-2) on the osteoclastic activity in periodontal ligament cells
Grantee:Bruno Nunes de França
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 15/11587-8 - Effect of the activation of protease activated receptor type 1 (PAR1)on the expression of osteogenic factors in periodontal ligament cells
Grantee:Emanuel da Silva Rovai
Support type: Scholarships in Brazil - Doctorate