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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of DNA mutations in gastric washes from gastric adenocarcinoma patients: Possible implications for liquid biopsies and patient follow-up

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Pizzi, Melissa Pool [1] ; Bartelli, Thais Fernanda [1] ; Pelosof, Adriane Graicer [2] ; Freitas, Helano Carioca [3, 1] ; Begnami, Maria Dirlei [4] ; Senda de Abrantes, Lais Lie [5] ; Sztokfisz, Claudia [2] ; Valieris, Renan [6] ; Knebel, Franciele Hinterholz [7] ; Vaz Coelho, Luiz Gonzaga [8] ; da Costa, Wilson Luiz [9] ; Coimbra, Felipe J. F. [9] ; da Silva, Israel Tojal [6] ; de Amorim, Maria Galli [1] ; Nunes, Diana Noronha [1] ; Dias-Neto, Emmanuel [1, 10]
Total Authors: 16
[1] AC Camargo Canc Ctr, Lab Med Genom, Tagua St, 440 Liberdade, Sao Paulo, SP - Brazil
[2] AC Camargo Canc Ctr, Endoscopy Sect, Sao Paulo, SP - Brazil
[3] AC Camargo Canc Ctr, Dept Clin Oncol, Sao Paulo, SP - Brazil
[4] AC Camargo Canc Ctr, Dept Pathol, Sao Paulo, SP - Brazil
[5] AC Camargo Canc Ctr, Int Res Ctr, Sao Paulo, SP - Brazil
[6] AC Camargo Canc Ctr, Lab Computat Biol, Sao Paulo, SP - Brazil
[7] Hosp Sirio Libanes, Ctr Oncol Mol, Sao Paulo, SP - Brazil
[8] Univ Fed Minas Gerais, Hosp Clin, Fac Med, Inst Alfa Gastroenterol, Belo Horizonte, MG - Brazil
[9] AC Camargo Canc Ctr, Dept Abdominal Surg, Sao Paulo, SP - Brazil
[10] Univ Sao Paulo, Fac Med, Inst Psiquiatria, Lab Neurosci Alzira Denise Hertzog Silva LIM 27, Sao Paulo, SP - Brazil
Total Affiliations: 10
Document type: Journal article
Source: International Journal of Cancer; v. 145, n. 4, p. 1090-1098, AUG 15 2019.
Web of Science Citations: 3

Whereas cancer patients have benefited from liquid biopsies, the scenario for gastric adenocarcinoma (GAC) is still dismal. We used next-generation deep sequencing of TP53-a highly mutated and informative gene in GAC-to assess mutations in tumor biopsies, plasma (PL) and stomach fluids (gastric wash-GW). We evaluated their potential to reveal tumor-derived mutations, useful for monitoring mutational dynamics at diagnosis, progression and treatment. Exon-capture libraries were constructed from 46 patients including tumor biopsies, GW and PL pre and post-treatment (196 samples), with high vertical coverage >8,000x. At diagnosis, we detected TP53 mutations in 15/46 biopsies (32.6%), 7/46 GW- (15.2%) and 6/46 PL-samples (13%). Biopsies and GW were concordant in 38/46 cases (82.6%) for the presence/absence of mutations and, furthermore, four GW-exclusive mutations were identified, suggesting tumor heterogeneity. Considering the combined analysis of GW and PL, TP53 mutations found in biopsies were also identified in 9/15 (60%) of cases, the highest detection level reported for GAC. Our study indicates that GW could be useful to track DNA alterations, especially if anchored to a comprehensive gene-panel designed for this malignancy. (AU)

FAPESP's process: 14/26897-0 - Epidemiology and genomics of gastric adenocarcinomas in Brazil
Grantee:Emmanuel Dias-Neto
Support type: Research Projects - Thematic Grants