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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of Enzymatic Activation of Bleaching Gels on Hydrogen Peroxide Degradation Rates, Bleaching Effectiveness, and Cytotoxicity

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Author(s):
Ortecho-Zuta, U. [1] ; de Oliveira Duque, C. C. [1] ; Leite, M. L. [1] ; Bordini, E. A. F. [1] ; Basso, F. G. [2] ; Hebling, J. [3] ; de Souza Costa, C. A. [2] ; Soares, D. G. [4]
Total Authors: 8
Affiliation:
[1] Univ Estadual Paulista UNESP, Dept Dent Mat & Prosthodont, Araraquara Sch Dent, Araraquara, SP - Brazil
[2] Univ Estadual Paulista UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil
[3] Univ Estadual Paulista UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP - Brazil
[4] Sao Paulo Univ USP, Dept Operat Dent Endodont & Dent Mat, Bauru Sch Dent, Bauru, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Operative Dentistry; v. 44, n. 4, p. 414-423, JUL-AUG 2019.
Web of Science Citations: 1
Abstract

Objectives: The aim of this study was to evaluate the effect of horseradish peroxidase (HRP) on the release of free radicals, bleaching effectiveness, and indirect cytotoxicity of a 35% hydrogen peroxide (HP) bleaching gel. Methods and Materials: First, HP degradation rates and free radical release were evaluated for 35% HP in contact or not with HRP (10 mg/mL). The bleaching gel associated or not with HRP was then applied (3 X 15 minutes) to enamel/dentin discs adapted to artificial pulp chambers, and the culture medium in contact with dentin surfaces (extract) was collected and exposed to cultured odontoblast-like cells. Membrane damage and viability of cells as well as oxidative stress were evaluated. Residual HP/free radical diffusion was quantified, and bleaching effectiveness (Delta E) was assessed. Unbleached discs served as negative controls. Results: The addition of HRP to the 35% HP bleaching gel enhanced the release of free radicals in comparison with plain HP gel. The 35% HP-mediated cytotoxicity significantly decreased with HRP in the bleaching gel and was associated with reduced HP/free radical diffusion through the enamel/dentin discs. Delta E values increased every bleaching session for HRP-containing gel relative to positive control, accelerating the whitening outcome. Conclusion: The enzymatic activation of a 35% HP bleaching gel with HRP accelerated HP degradation mediated by intensification of free radical release. This effect optimized whitening outcome as well as minimized residual HP and free radical diffusion through enamel and dentin, decreasing the harmful effects on odontoblast-like cells. (AU)

FAPESP's process: 16/15674-5 - Association of tissue engineering techniques for mineralized tissue regeneration under degenerative inflammatory stimulus: analysis on 3D-culture perfusion bioreactor and animal inflammatory models
Grantee:Diana Gabriela Soares dos Passos
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/21770-4 - Development of tooth bleaching products based on advanced oxidative process principles: analysis of esthetic and biologic effects
Grantee:Carlos Alberto de Souza Costa
Support Opportunities: Regular Research Grants