Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae)

Author(s):Show less -

Cavalcante Sassi, Francisco de Menezes ^[1] ; de Oliveira, Ezequiel Aguiar ^{[1, 2]} ; Carlos Bertollo, Luiz Antonio ^[1] ; Nirchio, Mauro ^[3] ; Hatanaka, Terumi ^[1] ; Ferreira Marinho, Manoela Maria ^{[1, 4]} ; Moreira-Filho, Orlando ^[1] ; Aroutiounian, Rouben ^[5] ; Liehr, Thomas ^[6] ; Al-Rikabi, Ahmed B. H. ^[6] ; Cioffi, Marcelo de Bello ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Lab Citogenet Peixes, BR-13565905 Sao Carlos, SP - Brazil

^[2] SEDUC MT, Secretaria Estado Educ Mato Grosso, BR-78049909 Cuiaba, MT - Brazil

^[3] Univ Tecn Machala, Fac Ciencias Agropecuarias, Machala 070151 - Ecuador

^[4] MZUSP, BR-04263000 Sao Paulo, SP - Brazil

^[5] Yerevan State Univ, Dept Genet & Cytol, Yerevan 0063 - Armenia

^[6] Univ Hosp Jena, Inst Human Genet, D-07747 Jena - Germany

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 12 JUN 2 2019.
• Web of Science Citations: 0

Abstract

We present the first cytogenetic data for Lebiasina bimaculata and L. melanoguttata with the aim of (1) investigating evolutionary events within Lebiasina and their relationships with other Lebiasinidae genera and (2) checking the evolutionary relationships between Lebiasinidae and Ctenoluciidae. Both species have a diploid number 2n = 36 with similar karyotypes and microsatellite distribution patterns but present contrasting C-positive heterochromatin and CMA(3)(+) banding patterns. The remarkable interstitial series of C-positive heterochromatin occurring in L. melanoguttata is absent in L. bimaculata. Accordingly, L. bimaculata shows the ribosomal DNA sites as the only GC-rich (CMA(3)(+)) regions, while L. melanoguttata shows evidence of a clear intercalated CMA(3)(+) banding pattern. In addition, the multiple 5S and 18S rDNA sites in L. melanogutatta contrast with single sites present in L. bimaculata. Comparative genomic hybridization (CGH) experiments also revealed a high level of genomic differentiation between both species. A polymorphic state of a conspicuous C-positive, CMA(3)(+), and (CGG)n band was found only to occur in L. bimaculata females, and its possible relationship with a nascent sex chromosome system is discussed. Whole chromosome painting (WCP) and CGH experiments indicate that the Lebiasina species examined and Boulengerella maculata share similar chromosomal sequences, thus supporting the relatedness between them and the evolutionary relationships between the Lebiasinidae and Ctenoluciidae families. (AU)

• FAPESP's process: 17/09321-5 - Miniature Characiformes (Teleostei: Ostariophysi): evolution, diversity and biogeography
• Grantee: Manoela Maria Marinho Koh
• Support type: BIOTA-FAPESP Program - Young Investigators Grants
• FAPESP's process: 18/22033-1 - An intercontinental approach for the investigation of chromosome evolution, genetic diversity and biogeography in the order Osteoglossiformes (Teleostei: Osteoglossomorpha). Part III
• Grantee: Marcelo de Bello Cioffi
• Support type: Regular Research Grants
• FAPESP's process: 18/24235-0 - Karyotype and sex chromosome evolution in Neotropical fishes. Part III.
• Grantee: Luiz Antonio Carlos Bertollo
• Support type: Regular Research Grants