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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Direct determination of anaerobe contributions to the energy metabolism of Trypanosoma cruzi by chip calorimetry

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Lerchner, Johannes [1] ; Sartori, Marina R. [2] ; Volpe, Pedro O. L. [3] ; Lander, Noelia [2] ; Mertens, Florian [1] ; Vercesi, Anibal E. [2]
Total Authors: 6
[1] TU Bergakad Freiberg, Inst Phys Chem, Leipziger Str 29, D-09599 Freiberg - Germany
[2] Univ Campinas UNICAMP, Dept Clin Pathol, BR-13083877 Campinas, SP - Brazil
[3] Univ Campinas UNICAMP, Inst Chem, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 411, n. 17, p. 3763-3768, JUL 2019.
Web of Science Citations: 0

We describe a chip calorimetric technique that allows the investigation of biological material under anoxic conditions in a micro-scale and in real time. Due to the fast oxygen exchange through the sample flow channel wall, the oxygen concentration inside the samples could be switched between atmospheric oxygen partial pressure to an oxygen concentration of 0.5% within less than 2h. Using this technique, anaerobic processes in the energy metabolism of Trypanosoma cruzi could be studied directly. The comparison of the calorimetric and respirometric response of T. cruzi cells to the treatment with the mitochondrial inhibitors oligomycin and antimycin A and the uncoupler FCCP revealed that the respiration-related heat rate is superimposed by strong anaerobic contributions. Calorimetric measurements under anoxic conditions and with glycolytic inhibitors showed that anaerobic metabolic processes contribute from 30 to 40% to the overall heat production rate. Similar basal and antimycin A heat rates with cells under anoxic conditions indicated that the glycolytic rates are independent of the oxygen concentration which confirms the absence of the Pasteur effect in Trypanosomes. (AU)

FAPESP's process: 17/17728-8 - Mitochondrial function and dysfunction: implications for aging and associated diseases
Grantee:Aníbal Eugênio Vercesi
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/08995-4 - Calcium signaling in trypanosomatids
Grantee:Noelia Marina Lander Manfredi
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/04613-8 - Real-time investigation of heat produced in the metabolic activity of erythrocytes of patients with sickle cell anemia in normoxia and anoxia conditions, using a segmented flow chip-calorimeter
Grantee:Pedro Luiz Onófrio Volpe
Support type: Research Grants - Visiting Researcher Grant - International
FAPESP's process: 17/05487-6 - Mitochondrial function and molecular markers associated with aging in the long-lived red-footed tortoise Chelonoidis carbonaria
Grantee:Marina Rincon Sartori
Support type: Scholarships in Brazil - Post-Doctorate