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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

S100A8 acts as an autocrine priming signal for heme-induced human M phi pro-inflammatory responses in hemolytic inflammation

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Silveira, Angelica A. A. [1] ; Mahon, Olwyn R. [2] ; Cunningham, Clare C. [2] ; Corr, Emma M. [2] ; Mendonca, Rafaela [1] ; Saad, Sara T. O. [1] ; Costa, Fernando F. [1] ; Dunne, Aisling [2] ; Conran, Nicola [1]
Total Authors: 9
[1] Univ Estadual Campinas, UNICAMP, Hematol Ctr, Campinas, SP - Brazil
[2] Trinity Coll Dublin, Trinity Biomed Sci Inst, Sch Biochem & Immunol, Dublin - Ireland
Total Affiliations: 2
Document type: Journal article
Source: Journal of Leukocyte Biology; v. 106, n. 1, p. 35-43, JUL 2019.
Web of Science Citations: 2

Intravascular hemolysis, in addition to reducing red cell counts, incurs extensive vascular inflammation and oxidative stress. One product of hemolysis, heme, is a potent danger associated molecular pattern (DAMP), activating leukocytes and inducing cytokine expression and processing, among other pro-inflammatory effects. We explored pathways by which heme-induced inflammation may be amplified under sterile conditions. Incubation of human M phi s, differentiated from CD14(+) cells, with heme induced time- and concentration-dependent gene and protein expression of S100A8, a myeloid cell-derived alarmin. Human M phi stimulation with recombinant S100A8, in turn, induced robust pro-IL-1 beta expression that was dependent upon NF-kappa B activation, gene transcription, and partially dependent upon TLR4-mediated signaling. Moreover, heme itself stimulated significant M phi pro-IL-1 beta gene and protein expression via an S100A8-mediated mechanism and greatly amplified S100A8-driven NLRP3 inflammasome-mediated IL-1 beta secretion. In vivo, induction of acute intravascular hemolysis in mice induced a rapid elevation of plasma S100A8 that could be abolished by hemopexin, a heme scavenger. Finally, plasma S100A8 levels were found to be significantly elevated in patients with the inherited hemolytic anemia, sickle cell anemia, when compared with levels in healthy individuals. In conclusion, we demonstrate that hemolytic processes are associated with S100A8 generation and that some of the inflammatory effects of heme may be amplified by autocrine S100A8 production. Findings suggest a mechanism by which hemolytic inflammation could be propagated via leukocyte priming by endogenous proteins, even in sterile inflammatory environments such as those that occur in the hemolytic diseases. S100A8 may represent a therapeutic target for reducing inflammation in hemolytic disorders. (AU)

FAPESP's process: 14/23170-1 - An investigation into the pro-inflammatory properties of heme in primary human macrophages
Grantee:Angélica Aparecida Antoniellis Silveira
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 14/19173-5 - Vascular inflammation: pathophysiological mechanisms of induction and pathways of cellular activation
Grantee:Nicola Amanda Conran Zorzetto
Support type: Regular Research Grants
FAPESP's process: 12/22048-2 - Investigation into the pro-inflammatory properties of TNF-alpha and heme in leukocytes in vitro and in vivo.
Grantee:Angélica Aparecida Antoniellis Silveira
Support type: Scholarships in Brazil - Doctorate