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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lytic Polysaccharide Monooxygenase from Aspergillus fumigatus can Improve Enzymatic Cocktail Activity During Sugarcane Bagasse Hydrolysis

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de Gouvea, Paula Fagundes [1] ; Gerolamo, Luis Eduardo [1] ; Bernardi, Aline Vianna [1] ; Soares Pereira, Lucas Matheus [1] ; Uyemura, Sergio Akira [2] ; Dinamarco, Taisa Magnani [1]
Total Authors: 6
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PROTEIN AND PEPTIDE LETTERS; v. 26, n. 5, p. 377-385, 2019.
Web of Science Citations: 1

Background: Lytic Polysaccharide Monooxygenases (LPMOs) are auxiliary accessory enzymes that act synergistically with cellulases and which are increasingly being used in second-generation bioethanol production from biomasses. Several LPMOs have been identified in various filamentous fungi, including Aspergillus fumigants. However, many LPMOs have not been characterized yet. Objective: To report the role of uncharacterized A. fumigatus AfAA9\_B LPMO. Methods: qRT-PCR analysis was employed to analyze the LPMO gene expression profile in different carbon sources. The gene encoding an AfAA9\_B (A fu4g07850) was cloned into the vector pET-28a(+), expressed in the E. coli strain Rosetta (TM) (DE3) pLysS, and purified by a Ni2+-nitrilotriacetic (Ni-NTA) agarose resin. To evaluate the specific LPMO activity, the purified protein peroxidase activity was assessed. The auxiliary LPMO activity was investigated by the synergistic activity in Celluclast 1.5L enzymatic cocktail. Results: LPMO was highly induced in complex biomass like sugarcane bagasse (SEB), Avicel (R) PH-101, and CM-cellulose. The LPMO gene encoded a protein comprising 250 amino acids, without a CBM domain. After protein purification, the AfAA9\_B molecular mass estimated by SDS-PAGE was 35 kDa. The purified protein specific peroxidase activity was 8.33+ 1.9 U g(-1). Upon addition to Celluclast 1.5L, Avicel (R) PH-101 and SEB hydrolysis increased by 18% and 22%, respectively. Conclusion: A. fumigatus LPMO is a promising candidate to enhance the currently available enzymatic cocktail and can therefore be used in second-generation ethanol production. (AU)

FAPESP's process: 18/10296-8 - Construction of a S. cerevisiae strain, by the CRISPR/Cas9 system, capable of expressing an LPMO, aiming at the improvement of the saccharification of lignocellulosic biomasses
Grantee:Paula Fagundes de Gouvêa Bizzi
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/19095-0 - Identification, characterization and functional expression of auxiliary activity enzymes (LPMOs) from Aspergillus fumigatus
Grantee:Taisa Magnani Dinamarco
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants