de-Oliveira, Marilia G.
Lira, Aline A. L.
Sgnotto, Fabio R.
Inoue, Amanda H. S.
Santos, Ludimila S.
Nakamatsu, Bernardo Y.
Duarte, Alberto J. S.
Victor, Jefferson R.
Total Authors: 9
 Univ Sao Paulo, Med Sch, Div Clin Dermatol, Lab Med Invest LIM 56, Av Dr Eneas de Carvalho Aguiar 500, 3rd Floor, BR-05403000 Sao Paulo - Brazil
 Univ Sao Paulo, Med Sch, Div Hematol, Sao Paulo - Brazil
 Laureate Int Univ, FMU, Div Environm Hlth, Sao Paulo - Brazil
 Univ Sao Paulo, Med Sch, Div Pathol, Sao Paulo - Brazil
 Univ Paris 05, Lab Immunoregulat & Immunopathol, INEM, CNRS UMR8253, INSERM, UMR1151, Paris - France
Total Affiliations: 5
CLINICAL AND EXPERIMENTAL ALLERGY;
Web of Science Citations:
Background The precise mechanism involved in the acquisition of the IL-17+ profile of gamma delta T cells, the ligands responsible for this change, and whether this default is acquired during intrathymic maturation need to be elucidated. Objective This study aimed to evaluate whether IL-17-producing gamma delta T cells are present in the airways of tolerant offspring from allergen-sensitized mothers and the possible implication of maternal IgG in the generation of these cells. Methods Female mice were immunized or not, and the allergic response, frequency of gamma delta T cell subsets and cytokine production of the offspring were analysed by flow cytometry. The effects of passive in vivo transfer of purified IgG were investigated in offspring. A translational approach was employed to analyse gamma delta T cells in the thymus and PBMCs from humans. Results Maternal immunization reduced the frequency of spontaneous IL-17-producing gamma delta T cells in the thymus, spleen and lung of offspring. This effect was mimicked by the in vivo treatment of females with purified IgG. IgG directly interacted with gamma delta T cell membranes. The modulatory effect of human IgG on human infant intrathymic and adult peripheral gamma delta T cells showed similarities to murine gamma delta T cells, which is rarely reported in the literature. Conclusions \& Clinical Relevance Together, our results reveal that IgG from potentially tolerant atopic mothers can influence offspring thymic IL-17-producing gamma delta T cell maturation. Furthermore, we suggest that IgG is an unprecedented modulatory factor of murine and human gamma delta T cells. These observations may support the future development of IgG-based immunoregulatory therapeutic strategies. (AU)