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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Maternal IgG impairs the maturation of offspring intrathymic IL-17-producing gamma delta T cells: Implications for murine and human allergies

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Author(s):
de-Oliveira, Marilia G. [1] ; Lira, Aline A. L. [1] ; Sgnotto, Fabio R. [2] ; Inoue, Amanda H. S. [1] ; Santos, Ludimila S. [3, 1] ; Nakamatsu, Bernardo Y. [3] ; Duarte, Alberto J. S. [4, 1] ; Leite-de-Moraes, Maria [5] ; Victor, Jefferson R. [3, 1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Med Sch, Div Clin Dermatol, Lab Med Invest LIM 56, Av Dr Eneas de Carvalho Aguiar 500, 3rd Floor, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Med Sch, Div Hematol, Sao Paulo - Brazil
[3] Laureate Int Univ, FMU, Div Environm Hlth, Sao Paulo - Brazil
[4] Univ Sao Paulo, Med Sch, Div Pathol, Sao Paulo - Brazil
[5] Univ Paris 05, Lab Immunoregulat & Immunopathol, INEM, CNRS UMR8253, INSERM, UMR1151, Paris - France
Total Affiliations: 5
Document type: Journal article
Source: CLINICAL AND EXPERIMENTAL ALLERGY; v. 49, n. 7, p. 1000-1012, JUL 2019.
Web of Science Citations: 2
Abstract

Background The precise mechanism involved in the acquisition of the IL-17+ profile of gamma delta T cells, the ligands responsible for this change, and whether this default is acquired during intrathymic maturation need to be elucidated. Objective This study aimed to evaluate whether IL-17-producing gamma delta T cells are present in the airways of tolerant offspring from allergen-sensitized mothers and the possible implication of maternal IgG in the generation of these cells. Methods Female mice were immunized or not, and the allergic response, frequency of gamma delta T cell subsets and cytokine production of the offspring were analysed by flow cytometry. The effects of passive in vivo transfer of purified IgG were investigated in offspring. A translational approach was employed to analyse gamma delta T cells in the thymus and PBMCs from humans. Results Maternal immunization reduced the frequency of spontaneous IL-17-producing gamma delta T cells in the thymus, spleen and lung of offspring. This effect was mimicked by the in vivo treatment of females with purified IgG. IgG directly interacted with gamma delta T cell membranes. The modulatory effect of human IgG on human infant intrathymic and adult peripheral gamma delta T cells showed similarities to murine gamma delta T cells, which is rarely reported in the literature. Conclusions \& Clinical Relevance Together, our results reveal that IgG from potentially tolerant atopic mothers can influence offspring thymic IL-17-producing gamma delta T cell maturation. Furthermore, we suggest that IgG is an unprecedented modulatory factor of murine and human gamma delta T cells. These observations may support the future development of IgG-based immunoregulatory therapeutic strategies. (AU)

FAPESP's process: 17/18558-9 - Influence of murine IgG repertoire on the maturation of B and gamma-deltaT cells with modulatory and/or regulatory potential in vitro
Grantee:Amanda Harumi Sabô Inoue
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 18/05181-7 - Translational evaluation of IgG effect upon the maturation of offspring thymus derived TCD4, TCD8, nTreg, gammadeltaT and e B cells.
Grantee:Alberto José da Silva Duarte
Support type: Regular Research Grants