The Impact of CO2 Laser Treatment and Acidulated Phosphate Fluoride on Enamel Demineralization and Biofilm Formation

Author(s)

de Araujo Loiola, Ana Barbara ^[1] ; Aires, Carolina Patricia ^[2] ; Curylofo-Zotti, Fabiana Almeida ^[1] ; Rodrigues Junior, Antonio Luiz ^[3] ; Souza-Gabriel, Aline Evangelista ^[1] ; Milori Corona, Silmara Aparecida ^[1]

Affiliation

^[1] Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Restorat Dent, Av Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, BR-14040904 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Social Med, BR-14049900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF LASERS IN MEDICAL SCIENCES; v. 10, n. 3, p. 200-206, SUM 2019.
• Web of Science Citations: 0

Abstract

Introduction: This study evaluated the impact of CO2 laser treatment and acidulated phosphate fluoride (APF) on enamel demineralization and biofilm formation, using in vitro and in situ designs. Methods: Demineralized enamel slabs were distributed among 8 groups: placebo, placebo + continuous CO2 laser, placebo + repeated CO2 laser, placebo + ultrapulsed CO2 laser, 1.23% APF, APF + continuous CO2 laser, APF + repeated CO2 laser and APF + ultrapulsed CO2 laser. In the in vitro study, 15 enamel slabs from each group were subjected to a pH-cycling regimen for 14 days. In the cross over in situ design, 11 volunteers wore palatal appliances with demineralized enamel slabs for 2 periods of 14 days each. Drops of sucrose solution were dripped onto enamel slabs 8x/day. Biofilms formed on slabs were collected and the colony-forming units (CFU) of Streptococcus mutans and Lactobacillus were determined. Results: For both in vitro and in situ studies, there was no significant difference between treatments (P > 0.05). However, all treatments increased microhardness of demineralized enamel (P < 0.05). After a further in situ cariogenic challenge, with the exception of the placebo, all treatments maintained microhardness values (P < 0.05). Microbiological analysis showed no difference in Streptococcus mutans (P > 0.05) or Lactobacillus (P > 0.05) counts between groups. Conclusion: The results suggest that APF gel combined with the CO2 laser, regardless of the pulse emission mode used, was effective in controlling enamel demineralization, but none of the tested treatments was able to prevent bacterial colonization. (AU)

• FAPESP's process: 12/01414-0 - Influency of mode emission pulse of CO2 laser and acidulated phosphate fluoride in the control of enamel demineralization: in vitro and in situ analysis
• Grantee: Silmara Aparecida Milori Corona
• Support type: Regular Research Grants
• FAPESP's process: 11/05363-9 - Influency of mode emission pulse of CO2 laser and acidulated phosphate fluoride in the control of enamel demineralization: in vitro and in situ analysis
• Grantee: Ana Bárbara de Araújo Loiola
• Support type: Scholarships in Brazil - Master