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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Pain inhibition through transplantation of fetal neuronal progenitors into the injured spinal cord in rats

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Author(s):
Batista, Chary M. [1] ; Mariano, Eric D. [1] ; Dale, Camila S. [2] ; Cristante, Alexandre F. [3] ; Britto, Luiz R. [4] ; Otoch, Jose P. [5] ; Teixeira, Manoel J. [1] ; Morgalla, Matthias [6] ; Lepski, Guilherme [7, 6]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Med, Dept Neurol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med, Dept Orthoped & Traumatol, Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil
[5] Univ Sao Paulo, Sch Med, Dept Surg, Sao Paulo - Brazil
[6] Eberhard Karls Univ Tubingen, Dept Neurosurg, Tubingen - Germany
[7] Univ Sao Paulo, Sch Med, Dept Psychiat, Sao Paulo - Brazil
Total Affiliations: 7
Document type: Journal article
Source: NEURAL REGENERATION RESEARCH; v. 14, n. 11, p. 2011-2019, NOV 2019.
Web of Science Citations: 1
Abstract

Neuropathic pain after spinal cord injury (SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy; nevertheless, the ideal cell type in terms of neurogenic potential and effectiveness against pain remains largely controversial. Here, we evaluated the ability of fetal neural stem cells (fNSC) to relieve chronic pain and, secondarily, their effects on motor recovery. Adult Wistar rats with traumatic SCI were treated, 10 days after injury, with intra-spinal injections of culture medium (sham) or fNSCs extracted from telencephalic vesicles (TV group) or the ventral medulla (VM group) of E/14 embryos. Sensory (von Frey filaments and hot plate) and motor (the Basso, Beattie, Bresnahan locomotor rating scale and inclined plane test) assessments were performed during 8 weeks. Thereafter, spinal cords were processed for immunofluorescence and transplanted cells were quantified by stereology. The results showed improvement of thermal hyperalgesia in the TV and VM groups at 4 and 5 weeks after transplantation, respectively. Moreover, mechanical allodynia improved in both the TV and VM groups at 8 weeks. No significant motor recovery was observed in the TV or VM groups compared with sham. Stereological analyses showed that similar to 70% of TV and VM cells differentiated into NeuN(+) neurons, with a high proportion of enkephalinergic and GABAergic cells in the TV group and enkephalinergic and serotoninergic cells in the VM group. Our study suggests that neuronal precursors from TV and VM, once implanted into the injured spinal cord, maturate into different neuronal subtypes, mainly GABAergic, serotoninergic, and enkephalinergic, and all subtypes alleviate pain, despite no significant motor recovery. The study was approved by the Animal Ethics Committee of the Medical School of the University of Sao Paulo (protocol number 033/14) on March 4, 2016. (AU)

FAPESP's process: 13/12056-0 - Myelopathic pain treatment by transplantation of neural progenitors in animal model of spinal cord injury
Grantee:Guilherme Alves Lepski
Support type: Regular Research Grants
FAPESP's process: 15/02154-0 - Assessment of neuropathic pain and sensorimotor function after stem cell transplantation in animal model of spinal cord injury
Grantee:Chary Ely Martin Marquez Batista
Support type: Scholarships in Brazil - Doctorate