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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A further unique chondroitin sulfate from the shrimp Litopenaeus vannamei with antithrombin activity that modulates acute inflammation

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Author(s):
Palhares, Lais C. G. F. [1, 2] ; Brito, Adriana S. [3, 2] ; de Lima, Marcelo A. [4, 5] ; Nader, Helena B. [4] ; London, James A. [1] ; Barsukov, Igor L. [1] ; Andrade, Giulianna P. V. [2] ; Yates, Edwin A. [1] ; Chavante, Suely F. [2]
Total Authors: 9
Affiliation:
[1] Univ Liverpool, Inst Integrat Biol, Dept Biochem, Liverpool L69 7ZB, Merseyside - England
[2] Univ Fed Rio Grande do Norte, Dept Bioquim, Programa Posgrad Bioquim & Biol Mol, Natal, RN - Brazil
[3] Univ Fed Rio Grande do Norte, Fac Ciencias Saude Trairi, Santa Cruz, RN - Brazil
[4] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo, SP - Brazil
[5] Keele Univ, Sch Life Sci, Mol & Struct Biosci, Huxley Bldg, Keele ST5 5BG, Staffs - England
Total Affiliations: 5
Document type: Journal article
Source: Carbohydrate Polymers; v. 222, OCT 15 2019.
Web of Science Citations: 0
Abstract

The detailed structure of a further Chondroitin Sulfate from Litopenaeus vannamei shrimp (sCS) is described. The backbone structure was established by H-1/C-13 NMR, which identified 3-O-sulfated GlcA, 4-O-sulfated GalNAc, 6-O-sulfated GalNAc, and 4,6-di-O-sulfated GalNAc residues. GlcA is linked to GalNAc 4,6 di S and GlcA 3S is linked to GalNAc 4S, GalNAc 4,6 di-S and GalNAc6S residues. The anticoagulant properties of this sCS were evaluated by activated partial thromboplastin time, anti-IIa, anti-Xa and anti-heparin cofactor II-mediated activities, and sCS failed to stabilise antithrombin in a fluoresence shift assay. The anti-inflammatory effect of sCS was explored using a model of acute peritonitis, followed by leukocyte count and measurement of the cytokines, IL-1 beta, IL-6 and TNF-alpha. The compound showed low clotting effects, but high anti-IIa activity and HCII-mediated thrombin inhibition. Its anti-inflammatory effect was shown by leukocyte recruitment inhibition and a decrease in pro-inflammatory cytokine levels. Although the biological role of sCS remains unknown, its properties indicate that it is suitable for studies of multi-potent molecules obtained from natural sources. (AU)

FAPESP's process: 17/14179-3 - Vesicle trafficking during heparan sulfate biosynthesis
Grantee:Ivarne Luis dos Santos Tersariol
Support type: Regular Research Grants
FAPESP's process: 15/03964-6 - Glycosaminoglycans and proteoglycans: interplay between structure and function
Grantee:Helena Bonciani Nader
Support type: Research Projects - Thematic Grants