| Full text | |
| Author(s): |
Palhares, Lais C. G. F.
[1, 2]
;
Brito, Adriana S.
[3, 2]
;
de Lima, Marcelo A.
[4, 5]
;
Nader, Helena B.
[4]
;
London, James A.
[1]
;
Barsukov, Igor L.
[1]
;
Andrade, Giulianna P. V.
[2]
;
Yates, Edwin A.
[1]
;
Chavante, Suely F.
[2]
Total Authors: 9
|
| Affiliation: | [1] Univ Liverpool, Inst Integrat Biol, Dept Biochem, Liverpool L69 7ZB, Merseyside - England
[2] Univ Fed Rio Grande do Norte, Dept Bioquim, Programa Posgrad Bioquim & Biol Mol, Natal, RN - Brazil
[3] Univ Fed Rio Grande do Norte, Fac Ciencias Saude Trairi, Santa Cruz, RN - Brazil
[4] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo, SP - Brazil
[5] Keele Univ, Sch Life Sci, Mol & Struct Biosci, Huxley Bldg, Keele ST5 5BG, Staffs - England
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Carbohydrate Polymers; v. 222, OCT 15 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
The detailed structure of a further Chondroitin Sulfate from Litopenaeus vannamei shrimp (sCS) is described. The backbone structure was established by H-1/C-13 NMR, which identified 3-O-sulfated GlcA, 4-O-sulfated GalNAc, 6-O-sulfated GalNAc, and 4,6-di-O-sulfated GalNAc residues. GlcA is linked to GalNAc 4,6 di S and GlcA 3S is linked to GalNAc 4S, GalNAc 4,6 di-S and GalNAc6S residues. The anticoagulant properties of this sCS were evaluated by activated partial thromboplastin time, anti-IIa, anti-Xa and anti-heparin cofactor II-mediated activities, and sCS failed to stabilise antithrombin in a fluoresence shift assay. The anti-inflammatory effect of sCS was explored using a model of acute peritonitis, followed by leukocyte count and measurement of the cytokines, IL-1 beta, IL-6 and TNF-alpha. The compound showed low clotting effects, but high anti-IIa activity and HCII-mediated thrombin inhibition. Its anti-inflammatory effect was shown by leukocyte recruitment inhibition and a decrease in pro-inflammatory cytokine levels. Although the biological role of sCS remains unknown, its properties indicate that it is suitable for studies of multi-potent molecules obtained from natural sources. (AU) | |
| FAPESP's process: | 17/14179-3 - Vesicle trafficking during heparan sulfate biosynthesis |
| Grantee: | Ivarne Luis dos Santos Tersariol |
| Support type: | Regular Research Grants |
| FAPESP's process: | 15/03964-6 - Glycosaminoglycans and proteoglycans: interplay between structure and function |
| Grantee: | Helena Bonciani Nader |
| Support type: | Research Projects - Thematic Grants |