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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Early diagnosis of Zika infection using a ZnO nanostructures-based rapid electrochemical biosensor

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Author(s):
Faria, Aline Macedo [1] ; Mazon, Talita [1]
Total Authors: 2
Affiliation:
[1] CTI, Ctr Tecnol Informacao Renato Archer, Rod D Pedro 1, KM 143-6, BR-13069901 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Talanta; v. 203, p. 153-160, OCT 1 2019.
Web of Science Citations: 0
Abstract

In this work, we report a develop of electrochemical immunosensor based on ZnO nanostructures immobilized with ZIKV-NS1 antibody on Printed Circuit Board (PCB). ZnO nanostructures were grown on PCB by chemical bath deposition (CDB) and characterized by SEM. ZIKV-NS1 antibody was immobilized on the ZnO nanostructures surface via cystamine and glutaraldehyde. The samples were characterized by Immunofluorescence Confocal Microscopy and FTIR to identify the immobilization of the antibody to the sensor board. The analytical responses of the immunosensor were evaluated by Cyclic Voltammetry (CV). The biosensor developed here allows rapid detection of Zika virus in undiluted urine, without cross reactive with DENV-NS1 antigen, with linear range 0.1 ng mL(-1) to 100 ng mL(-1). The limit of detection is lower than 1.00 pg mL(-1). The biosensor is portable, cost-effectiveness, and simple to use, which makes it ideal for point-of-care applications. (AU)

FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
Grantee:Elson Longo da Silva
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC