Effects of different culture media, cell densities and adhesion periods on stem cells from human exfoliated deciduous teeth after photobiomodulation

Author(s):Show less -

Oliveira Prado, Marie Tavares ^[1] ; Ribeiro Vitor, Luciana Lourengo ^[1] ; Lourenco Neto, Natalino ^[1] ; Teixeira Marques, Nadia Carolina ^[2] ; Sakai, Vivien Thiemy ^[3] ; Rios, Daniela ^[1] ; Cruvinel, Thiago ^[1] ; Oliveira, Rodrigo Cardoso ^[4] ; Santos, Carlos Ferreira ^[3] ; Andrade Moreira Machado, Maria Aparecida ^[1] ; Oliveira, Thais Marchini ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Pediat Dent Orthodont & Publ Hlth, Bauru Sch Dent, 975 Ave Dr Octavio Pinheiro Brisolla, BR-17012901 Bauru, SP - Brazil

^[2] Jose do Rosario Vellano Univ, Dept Pediat Dent, Sch Dent, Rd MG 179 Km 0, BR-37130000 Alfenas, MG - Brazil

^[3] Univ Fed Alfenas, Dept Clin & Surg, Sch Dent, 700 Str Gabriel Monteiro da Silva, BR-37130000 Alfenas, MG - Brazil

^[4] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, 975 Ave Dr Octavio Pinheiro Brisolla, BR-17012901 Bauru, SP - Brazil

• Document type: Journal article
• Source: LASER PHYSICS LETTERS; v. 16, n. 9 SEP 2019.
• Web of Science Citations: 0

Abstract

This study aimed to evaluate different culture media, cell densities and adhesion periods on stem cells from human exfoliated deciduous teeth (SKIED) after photobiomodulation (PBM). SHED was cultured in either MEMa or DMEM with 10% Fetal Bovine Serum (FBS), let to adhere for 24h or 48h, submitted to 1%FBS, and irradiated. The following densities were tested: 2 x 10(3) (G1, G2), 1 x 10(4) (G3, G4), and 2 x 10(4) (G5, G6). PBM was applied at 20 mW for 10s (5.0 J cm(-2)). Control groups were not irradiated (G2, G4, G6). MTT assay evaluated cell viability at 6 h, 12 h and 24h. The comparison between different culture media revealed statistically significant differences among periods (p < 0.05) and no difference among groups at all periods. The statistical analysis showed the interaction between cell density, period, and adhesion (p < 0.001). The comparison between adhesion periods revealed that after PBM, at the first period G3 and G4 showed greater cell viability at 48h of adhesion, while G5 and G6 had greater cell viability at 24h of adhesion than at 48h at both periods. G3, G5 and G6 had greater cell viability 24h after PBM at 24h of adhesion than at 48 h. Based on this study, the culture medium type MEM alpha or DMEM did not change the SKIED viability after PBM. The adhesion period of 24h, 6 and 24h after PBM, the SHED density of 2 x 10(4) exhibited greater viability. However, with the adhesion period of 48h, this SHED density showed a decreasing in viability, 6 and 24h after PBM. Higher cell density (1 x 10(4) and 2 x 10(4)) and shorter adhesion period (24h) led to greater cell viability after PBM. (AU)

• FAPESP's process: 15/19696-0 - FGF2, VEGF, DSPP, and DMP-1 expression in cells of the pulp deciduous teeth with a low intensity laser
• Grantee: Mariel Tavares de Oliveira Prado Bergamo
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/18886-5 - Low intensity laser effects of different energy densities in cells of the pulp of human deciduous teeth
• Grantee: Thais Marchini de Oliveira Valarelli
• Support Opportunities: Regular Research Grants