Assessing the cytotoxic potential of glycoalkaloidic extract in nanoparticles against bladder cancer cells

Author(s):Show less -

Miranda, Mariza A. ^[1] ; Marcato, Priscyla D. ^[1] ; Carvalho, Ivana P. S. ^[1] ; Silva, Leticia B. ^[1] ; Ribeiro, Diego L. ^[2] ; Amaral, Robson ^[1] ; Swiech, Kamilla ^[1] ; Bastos, Jairo K. ^[1] ; Paschoal, Jonas A. R. ^[1] ; dos Reis, Rodolfo B. ^[3] ; Bentley, Maria V. L. B. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Div Urol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Pharmacy and Pharmacology; v. 71, n. 10 AUG 2019.
• Web of Science Citations: 0

Abstract

Objective This study proposed to use the nanotechnology to deliver glycoalkaloidic extract (AE) to bladder cancer cells, evaluating their activity in 2D and 3D models and the biological mechanism of cell death. Methods NPs were prepared by nanoprecipitation method using polylactic acid (PLA) and characterized considering their size, charge, particle concentration and stability. The cytotoxicity was evaluated in 2D and 3D model, and the apoptosis and cell cycle were investigated using flow cytometry. Key findings NPs loading AE (NP-AE) had diameter around 125 +/- 6 nm (PdI <0.1) and negative charge. The encapsulation efficiency of SM and SS was higher than 85% for both compounds. The obtained formulation showed a significant in-vitro cytotoxic effect against RT4 cells in a dose-dependent manner with IC50 two fold lower than the free AE. The cytotoxic effect of NP-AE was mediated by apoptosis and cell cycle arrested in the S phase. RT4 cells cultured under 3D conditions exhibited a higher resistance to the treatments (IC50 similar to three fold higher than in 2D cell culture). Conclusion The NP-AE might be a promising nanocarrier to load and deliver glycoalkaloids against bladder cancer. (AU)

• FAPESP's process: 14/50928-2 - INCT 2014: Pharmaceutical Nanotechnology: a transdisciplinary approach
• Grantee: Maria Vitória Lopes Badra Bentley
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/14665-0 - Development of functionalized nanoparticles with folate for the delivery of glycoalkaloids of Solanum lycocarpum and biological evaluation using 2D and 3D cell culture models
• Grantee: Mariza Abreu Miranda
• Support Opportunities: Scholarships in Brazil - Post-Doctoral