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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Increased whitening efficacy and reduced cytotoxicity are achieved by the chemical activation of a highly concentrated hydrogen peroxide bleaching gel

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Author(s):
Soares, Diana Gabriela [1] ; Marcomini, Natalia [2] ; de Oliveira Duque, Carla Caroline [3] ; Bordini, EsterAlves Ferreira [3] ; Zuta, Uxua Ortecho [3] ; Basso, Fernanda Goncalves [4] ; Hebling, Josimeri [5] ; de Souza Costa, Carlos Alberto [4]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Odontol Bauru, Dept Dentist Endodontia & Mat Odontol, Bauru, SP - Brazil
[2] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, Dept Dentist Restauradora, Araraquara, SP - Brazil
[3] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, Dept Mat Dent & Protese, Araraquara, SP - Brazil
[4] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, Dept Fisiol & Patol, Araraquara, SP - Brazil
[5] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, Dept Clin Infantil, Araraquara, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Applied Oral Science; v. 27, 2019.
Web of Science Citations: 1
Abstract

Abstract Objective This study was designed for the chemical activation of a 35% hydrogen peroxide (H2O2) bleaching gel to increase its whitening effectiveness and reduce its toxicity. Methodology First, the bleaching gel - associated or not with ferrous sulfate (FS), manganese chloride (MC), peroxidase (PR), or catalase (CT) - was applied (3x 15 min) to enamel/dentin discs adapted to artificial pulp chambers. Then, odontoblast-like MDPC-23 cells were exposed for 1 h to the extracts (culture medium + components released from the product), for the assessment of viability (MTT assay) and oxidative stress (H2DCFDA). Residual H2O2 and bleaching effectiveness (DE) were also evaluated. Data were analyzed with one-way ANOVA complemented with Tukey’s test (n=8. p<0.05). Results All chemically activated groups minimized MDPC-23 oxidative stress generation; however, significantly higher cell viability was detected for MC, PR, and CT than for plain 35% H2O2 gel. Nevertheless, FS, MC, PR, and CT reduced the amount of residual H2O2 and increased bleaching effectiveness. Conclusion Chemical activation of 35% H2O2 gel with MC, PR, and CT minimized residual H2O2 and pulp cell toxicity; but PR duplicated the whitening potential of the bleaching gel after a single 45-minute session. (AU)

FAPESP's process: 15/21770-4 - Development of tooth bleaching products based on advanced oxidative process principles: analysis of esthetic and biologic effects
Grantee:Carlos Alberto de Souza Costa
Support Opportunities: Regular Research Grants