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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of the sensory, affective, cognitive, biochemical, and neuronal alterations in a modified chronic constriction injury model of neuropathic pain in mice

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Author(s):
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Medeiros, Priscila [1, 2, 3, 4, 5] ; de Freitas, Renato Leonardo [1, 2, 3, 4, 5, 6] ; Boccella, Serena [1] ; Iannotta, Monica [1] ; Belardo, Carmela [1] ; Mazzitelli, Mariacristina [1] ; Romano, Rosaria [1] ; De Gregorio, Danilo [7] ; Coimbra, Norberto Cysne [2, 3, 4, 5] ; Palazzo, Enza [1] ; Maione, Sabatino [1]
Total Authors: 11
Affiliation:
[1] Univ Campania Luigi Vanvitelli, Dept Expt Med, Div Pharmacol, Naples - Italy
[2] Univ Sao Paulo, FMRP, Ribeirao Preto Med Sch, Dept Surg & Anat, Lab Neurosci Pain & Emot, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, FMRP, Ribeirao Preto Med Sch, Dept Surg & Anat, Multiuser Ctr Neuroelectrophysio, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, FMRP, Dept Pharmacol, Lab Neuroanat & Neuropsychobiol, Ribeirao Preto - Brazil
[5] Behav Neurosci Inst INeC, Ribeirao Preto - Brazil
[6] Fed Univ Alfenas UNIFAL, Biomed Sci Inst, Alfenas, MG - Brazil
[7] McGill Univ, Dept Psychiat, Neurobiol Psychiat Unit, Montreal, PQ - Canada
Total Affiliations: 7
Document type: Journal article
Source: Journal of Neuroscience Research; AUG 2019.
Web of Science Citations: 0
Abstract

The chronic constriction injury (CCI) of the sciatic nerve is a nerve injury-based model of neuropathic pain (NP). Comorbidities of NP such as depression, anxiety, and cognitive deficits are associated with a functional reorganization of the medial prefrontal cortex (mPFC). Here, we have employed an adapted model of CCI by placing one single loose ligature around the sciatic nerve in mice for investigating the alterations in sensory, motor, affective, and cognitive behavior and in electrophysiological and biochemical properties in the prelimbic division (PrL) of the mPFC. Our adapted model of CCI induced mechanical allodynia, motor, and cognitive impairments and anxiety- and depression-like behavior. In the PrL division of mPFC was observed an increase in GABA and a decrease in d-aspartate levels. Moreover an increase in the activity of neurons responding to mechanical stimulation with an excitation, mPFC (+), and a decrease in those responding with an inhibition, mPFC (-), was found. Altogether these findings demonstrate that a single ligature around the sciatic nerve was able to induce sensory, affective, cognitive, biochemical, and functional alterations already observed in other neuropathic pain models and it may be an appropriate and easily reproducible model for studying neuropathic pain mechanisms and treatments. (AU)

FAPESP's process: 14/11869-0 - Multi-use equipment approved in grant 2013/12916-0: deep brain stimulation (DBS) (Thomas mini matrix system - Thomas recording GmbH® - Winchester Strasse - Giessen - Germany)
Grantee:Renato Leonardo de Freitas
Support type: Multi-user Equipment Program
FAPESP's process: 13/12916-0 - Role of endocannabinoid, glutamatergic and endovanilloid systems of medial prefrontal cortex in neuropathic pain model and investigation of neurological disorders and chronic pain comorbidity
Grantee:Renato Leonardo de Freitas
Support type: Research Grants - Young Investigators Grants