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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetically Engineered Proteins to Improve Biomass Conversion: New Advances and Challenges for Tailoring Biocatalysts

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Author(s):
Ribeiro, Lucas Ferreira [1] ; Amarelle, Vanesa [2] ; Alves, Luana de Fatima [3] ; Viana de Siqueira, Guilherme Marcelino [3] ; Lovate, Gabriel Lencioni [3] ; Borelli, Tiago Cabral [1] ; Guazzaroni, Maria-Eugenia [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto - Brazil
[2] Biol Res Inst Clemente Estable, Dept Microbial Biochem & Genom, Montevideo 11600 - Uruguay
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto - Brazil
Total Affiliations: 3
Document type: Review article
Source: Molecules; v. 24, n. 16 AUG 2019.
Web of Science Citations: 0
Abstract

Protein engineering emerged as a powerful approach to generate more robust and efficient biocatalysts for bio-based economy applications, an alternative to ecologically toxic chemistries that rely on petroleum. On the quest for environmentally friendly technologies, sustainable and low-cost resources such as lignocellulosic plant-derived biomass are being used for the production of biofuels and fine chemicals. Since most of the enzymes used in the biorefinery industry act in suboptimal conditions, modification of their catalytic properties through protein rational design and in vitro evolution techniques allows the improvement of enzymatic parameters such as specificity, activity, efficiency, secretability, and stability, leading to better yields in the production lines. This review focuses on the current application of protein engineering techniques for improving the catalytic performance of enzymes used to break down lignocellulosic polymers. We discuss the use of both classical and modern methods reported in the literature in the last five years that allowed the boosting of biocatalysts for biomass degradation. (AU)

FAPESP's process: 16/18827-7 - Integrating protein engineering and synthetic biology to develop new high-throughput selection systems
Grantee:Lucas Ferreira Ribeiro
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 15/04309-1 - Novel approaches to improve functional screening of biocatalysts in metagenomic libraries
Grantee:María Eugenia Guazzaroni
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 18/18158-3 - Search for determinants of antibiotic resistance in the interior of São Paulo using functional metagenomics
Grantee:Gabriel Lencioni Lovate
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 18/07261-8 - Expanding acid resistance in bacteria using synthetic circuits
Grantee:Guilherme Marcelino Viana de Siqueira
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 19/00390-0 - Resistome and mobilome in genomes of bacteria from diverse Brazilian regions
Grantee:Tiago Cabral Borelli
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 16/06323-4 - New tools for improving metagenomic cellulase screenings
Grantee:Luana de Fátima Alves
Support Opportunities: Scholarships in Brazil - Doctorate