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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inflammation and kidney injury attenuated by prior intake of Brazil nuts in the process of ischemia and reperfusion

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Maria Fernanda Ribeiro Cury ; Estéfany Queiroz Olivares ; Renata Correia Garcias ; Giovana Queda Toledo ; Natassia Alberici Anselmo ; Leticia Colombo Paskakulis ; Fernanda Fortuci Resende Botelho ; Natiele Zanardo Carvalho ; Analice Andreoli da Silva ; Camila Agren ; Carla Patrícia Carlos
Total Authors: 11
Document type: Journal article
Source: J. Bras. Nefrol.; v. 40, n. 4, p. 312-318, Dez. 2018.

ABSTRACT Introduction: Ischemia and reperfusion (IR) is a process inherent to the procedures involved in the transplantation of organs that causes inflammation, cell death and cell injury, and may lead to rejection of the graft. It is possible that the anti-inflammatory properties of the Brazil nuts (BN) can mitigate the renal injury caused by IR. Objective: To investigate whether the previous intake of BN reduces the expression of markers of inflammation, injury, and cell death after renal IR. Methods: Male Wistar rats were distributed into six groups (N = 6/group): SHAM (control), SHAM treated with 75 or 150 mg of BN, IR, and IR treated with 75 or 150 mg of BN. The IR procedure consisted of right nephrectomy and occlusion of the left renal artery with a non-traumatic vascular clamp for 30 min. BN was given daily from day 1 to 7 before surgery (SHAM or IR), and maintained until sacrifice (48 h after surgery). Inflammation was evaluated by renal expression of COX-2 and TGF-β, injury by the expression of vimentin, and cell death by apoptosis through caspase-3 expression (immunohistochemistry). Results: Pretreatment with 75 mg of BN reduced renal expression of the COX-2, TGF-β, vimentin, and caspase-3. The dose of 150 mg caused increased expression of COX-2. Conclusion: In experimental IR, the damage can be minimized with a prior low-dose intake of BN, improving inflammation, injury, and cell death. (AU)

FAPESP's process: 15/25616-0 - Oxidative stress and renal inflammation in rats submitted to ischemia-reperfusion and antioxidant treatment
Grantee:Renata Correia Garcias
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/07138-9 - Kidney expression of markers of injury and apoptosis in rats subjected to renal ischemia and reperfusion and treatment with Brazil nuts
Grantee:Estéfany Queiroz Olivares
Support type: Scholarships in Brazil - Scientific Initiation