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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Silk fibroin films stabilizes and releases bioactive insulin for the treatment of corneal wounds

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Author(s):
Cubayachi, Camila [1] ; Lemos, Camila Nunes [1] ; Pereira, Francieli [1] ; Dias, Karina [1] ; Herculano, Rondinelli Donizetti [2] ; de Freitas, Osvaldo [1] ; Lopez, Renata F. V. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil
[2] Sao Paulo State Univ, Sch Pharmaceut Sci, Araraquara, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: EUROPEAN POLYMER JOURNAL; v. 118, p. 502-513, SEP 2019.
Web of Science Citations: 2
Abstract

Insulin (INS) is a protein that plays a crucial role in many cellular functions, including wound healing processes. However, its topical ocular administration is a challenge, mainly because of INS degradation and low retention time, which can significantly reduce its bioavailability. The objective of this work was to develop films based on silk fibroin (SF), a natural polymer with anti-inflammatory properties, containing INS aimed at the sustained release of a bioactive INS in the eye for the treatment of corneal wounds. SF films containing 100 IU/cm(2) INS and glycerin as plasticizer were prepared by casting. They were homogeneous, transparent, permeable to water vapor, with low swelling index and high mechanical resistance. Fourier transformed infrared spectroscopy and differential scanning calorimetry analyses suggested the beta-sheet/Silk II conformation of SF and the occurrence of non-covalent interactions between INS and SF. Scanning electron microscopy suggested that the INS was embedded inside the film in its native globular form. In fact, the INS released from the film maintained its native conformation, as observed by circular dichroism, in addition to conserving its biological activity in vivo, reducing the blood glycemia of Wistar rats. Sustained release, of approximately 1 IU/cm(2)/h(1/2) of INS per hour, following a burst release, suggests that the film could be effective in the recovery of injured corneal epithelium, avoiding the need for the various daily administrations. Therefore, SF films demonstrated potential to transport and release INS in a sustained manner, a promising strategy for the treatment of corneal wounds. (AU)

FAPESP's process: 14/19018-0 - Evaluation of formulations based on silk fibroin and insulin for topical treatment of ocular wounds
Grantee:Camila Cubayachi
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/22451-7 - Sustained drug delivery systems targeting the epithelial tissue
Grantee:Renata Fonseca Vianna Lopez
Support type: Research Projects - Thematic Grants