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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genome-resolved metagenomics of sugarcane vinasse bacteria

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Cassman, Noriko A. [1] ; Lourenco, Kesia S. [2, 1] ; do Carmo, Janaina B. [3] ; Cantarella, Heitor [2] ; Kuramae, Eiko E. [1]
Total Authors: 5
[1] KNAW, Netherlands Inst Ecol NIOO, Dept Microbial Ecol, Wageningen - Netherlands
[2] Agron Inst Campinas, Soils & Environm Resources Ctr, POB 28, BR-13012970 Campinas, SP - Brazil
[3] Univ Fed Sao Carlos, Environm Sci Dept, BR-18052780 Soro Carba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 7

Background: The production of 1 L of ethanol from sugarcane generates up to 12 L of vinasse, which is a liquid waste containing an as-yet uncharacterized microbial assemblage. Most vinasse is destined for use as a fertilizer on the sugarcane fields because of the high organic and K content; however, increased N2O emissions have been observed when vinasse is co-applied with inorganic N fertilizers. Here we aimed to characterize the microbial assemblage of vinasse to determine the gene potential of vinasse microbes for contributing to negative environmental effects during fertirrigation and/or to the obstruction of bioethanol fermentation. Results: We measured chemical characteristics and extracted total DNA from six vinasse batches taken over 1.5 years from a bioethanol and sugar mill in Sao Paulo State. The vinasse microbial assemblage was characterized by low alpha diversity with 5-15 species across the six vinasses. The core genus was Lactobacillus. The top six represented bacterial genera across the samples were Lactobacillus, Megasphaera and Mitsuokella (Phylum Firmicutes, 35-97% of sample reads); Arcobacter and Alcaligenes (Phylum Proteobacteria, 0-40%); Dysgonomonas (Phylum Bacteroidetes, 0-53%); and Bifidobacterium (Phylum Actinobacteria, 0-18%). Potential genes for denitrification but not nitrification were identified in the vinasse metagenomes, with putative nirK and nosZ genes the most represented. Binning resulted in 38 large bins with between 36.0 and 99.3% completeness, and five small mobile element bins. Of the large bins, 53% could be classified at the phylum level as Firmicutes, 15% as Proteobacteria, 13% as unknown phyla, 13% as Bacteroidetes and 6% as Actinobacteria. The large bins spanned a range of potential denitrifiers; moreover, the genetic repertoires of all the large bins included the presence of genes involved in acetate, CO2, ethanol, H2O2, and lactose metabolism; for many of the large bins, genes related to the metabolism of mannitol, xylose, butyric acid, cellulose, sucrose, ``3-hydroxy{''} fatty acids and antibiotic resistance were present based on the annotations. In total, 21 vinasse bacterial draft genomes were submitted to the genome repository. Conclusions: Identification of the gene repertoires of vinasse bacteria and assemblages supported the idea that organic carbon and nitrogen present in vinasse together with microbiological variation of vinasse might lead to varying patterns of N2O emissions during fertirrigation. Furthermore, we uncovered draft genomes of novel strains of known bioethanol contaminants, as well as draft genomes unknown at the phylum level. This study will aid efforts to improve bioethanol production efficiency and sugarcane agriculture sustainability. (AU)

FAPESP's process: 13/50365-5 - Microbial networks in control of greenhouse gases emissions in biobased agriculture - MiniBag
Grantee:Heitor Cantarella
Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 13/12716-0 - Greenhouse gas flows (CO2, N2O and CH4) from soil with sugarcane as affected by regular or concentrate vinasse at different application times
Grantee:Késia Silva Lourenço
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/24141-5 - Microbial networks in control of greenhouse gases emissions from soil with sugarcane as affected by regular or concentrate vinasse
Grantee:Késia Silva Lourenço
Support type: Scholarships abroad - Research Internship - Doctorate