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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proinflammatory profile of neonatal monocytes induced by microbial ligands is downmodulated by histamine

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Calvielli Castelo Branco, Anna Claudia [1, 2] ; Pereira, Natalli Zanete [2] ; Yamada Yoshikawa, Fabio Seiti [2] ; da Silva Oliveira, Luanda Mara [2] ; Emidio Teixeira, Franciane Mouradian [1, 2] ; Oliveira, Luana de Mendonca [2] ; Pietrobon, Anna Julia [1, 2] ; Torrealba, Marina Passos [2] ; de Lima, Josenilson Feitosa [2] ; da Silva Duarte, Alberto Jose [2] ; Sato, Maria Notomi [1, 2]
Total Authors: 11
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Med Sch, Trop Med Inst Sao Paulo, Dept Dermatol, Lab Med Invest, LIM 56, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, SEP 23 2019.
Web of Science Citations: 0

Although the neonatal period is characterized by relative immunological immaturity, an inflammatory response due to Toll-like receptor (TLR) activation is observed. Histamine may be one of the factors playing a role in restraining inflammation during the early stages of life. Therefore, we evaluated the responsiveness of human cord blood cells to TLR4 agonists and the immunomodulatory function of histamine in the inflammatory response. Compared with adults, mononuclear cells (MNCs) from newborns (NBs) exhibit impaired production of IFN-gamma-inducible chemokines, such as CXCL10 and CXCL9, upon lipopolysaccharide (LPS) stimulation. Notably, LPS induced a 5-fold increase in CCL2 secretion in NBs. Evaluation of the effect of histamine on LPS-induced CCL2 secretion showed an inhibitory effect in the majority of adults, whereas this effect was detectable in all NBs. Histamine receptor (HR) blockage revealed partial involvement of H1R, H2R and H4R in LPS-induced CCL2 inhibition in MNCs from both NBs and adults. As monocytes are the main type of mononuclear cell that produces CCL2, we evaluated genes related to TLR signaling upon LPS stimulation. Monocytes from NBs showed up-regulation of genes associated with JAK/STAT/NF-kappa B and IFN signaling. Some differentially expressed genes encoding proinflammatory factors were preferentially detected in LPS-activated monocytes from NBs, and markedly down-regulated by histamine. The immunomodulatory role of histamine on CCL2 and CXCL8 was detected at the transcript and protein levels. Our findings show that NBs have enhanced CCL2 responsiveness to LPS, and that histamine acts in immune homeostasis during the neonatal period to counterbalance the robustness of TLR stimulation. (AU)

FAPESP's process: 16/24680-9 - Evaluation of inflammation of human villous trophoblasts in Zika and dengue virus
Grantee:Anna Cláudia Calvielli Castelo Branco
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/16524-6 - Effect of histamine on the activation of dendritic cells and CD4+T cells mediated by the agonists of toll-like receptors in the newborn
Grantee:Maria Notomi Sato
Support type: Regular Research Grants