Use of pregnancy-associated plasma protein-A during oocyte in vitro maturation increases IGF-1 and affects the transcriptional profile of cumulus cells and embryos from Nelore cows

Author(s):Show less -

Giroto, Alan B. ^[1] ; Fontes, Patricia K. ^[2] ; Franchi, Fernanda F. ^[2] ; dos Santos, Priscila H. ^[2] ; Razza, Eduardo M. ^[2] ; Nogueira, Marcelo F. G. ^[3] ; Maioli, Marcos A. ^[4] ; Nogueira, Guilherme P. ^[4] ; Nunes, Giovana B. ^[4] ; Mingoti, Gisele Z. ^[4] ; Mareco, Edson A. ^[1] ; Castilho, Anthony C. S. ^[1]

Affiliation

^[1] Univ Oeste Paulista, Presidente Prudente, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Dept Farmacol, Botucatu, SP - Brazil

^[3] Univ Estadual Paulista ESP, Dept Ciencias Biol, Assis, SP - Brazil

^[4] Univ Estadual Paulista UNESP, Dept Apoio Prod & Saude Anim, Aracatuba, SP - Brazil

• Document type: Journal article
• Source: Molecular Reproduction and Development; v. 86, n. 11, p. 1694-1704, NOV 2019.
• Web of Science Citations: 0

Abstract

Insulin-like growth factor 1 (IGF-1) activity is established by the regulation of IGF binding protein activity, which blocks IGF-1 functions, whereas pregnancy-associated plasma protein-A (PAPP-A) improves IGF-1 bioavailability and facilitates binding to IGF receptors. To further extend our understanding of the effect of exogenous PAPP-A on bovine embryo production, we added this protein during in vitro maturation of cumulus-oocyte complexes (COCs); moreover, we assessed its effects on IGF-1 quantity in the maturation medium, embryonic yield and postwarming survival, blastocyst quality, and transcript abundance. Bovine COCs were matured in a serum-free medium, either with PAPP-A supplementation (100 ng/ml) or without (control). The treatment group produced higher IGF-1 concentrations in the maturation medium; however, showed no difference on cleavage, blastocysts rates, and embryonic survival 3 and 24 hr postcryopreservation. Regarding gene expression, VNN1 was upregulated, whereas AGPAT9, FASN, EGFR, HAS2, and IMPDH1 were downregulated in PAPP-A treated. PAPP-A treated, CPT2, DNMT3A, and TFAM were upregulated, whereas ATF4 and IFITM3 were downregulated. We concluded that although the addition of PAPP-A did not affect embryo yield and blastocyst survival, higher IGF-1 levels may affect embryo competence through differential expression of genes involved in lipid metabolism, oocyte competence, and mitochondrial function. (AU)

• FAPESP's process: 16/22812-5 - Cumulus-oocyte complexes in vitro maturation from Holstein cows with pregnancy-associated plasm protein-A: effects on embryo production and quality
• Grantee: Alan Brunholi Giroto
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/04505-5 - Characterization of microRNAs (miRNAs) in bovine antral follicles from Nelore cows: involvement during ovarian follicle cells differentiation and regulation of LH receptor (LHR) expression
• Grantee: Anthony César de Souza Castilho
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
• Grantee: Marcelo Fábio Gouveia Nogueira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/11480-3 - Characterization of microRNAs (miRNAs) in bovine antral follicles from Nelore cattle: involvement during ovarian follicle cells differentiation and regulation of LH receptor (LHr) expression
• Grantee: Anthony César de Souza Castilho
• Support Opportunities: Research Grants - Young Investigators Grants