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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impact of calcium aluminate cement with additives on dental pulp-derived cells

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Nadyne Saab Messias ; Gabriela Grisote ; Antonio Secco Martorano ; Roger Rodrigo Fernandes ; Ivone Regina de Oliveira ; Karina Fittipaldi Bombonato-Prado ; Paulo Tambasco de Oliveira ; Larissa Moreira Spinola de Castro-Raucci
Total Authors: 8
Document type: Journal article
Source: Journal of Applied Oral Science; v. 28, p. -, 2020.
Web of Science Citations: 0

Abstract Calcium aluminate cement (CAC) has been highlighted as a promising alternative for endodontic use aiming at periapical tissue repair. However, its effects on dental pulp cells have been poorly explored. Objective: This study assessed the impact of calcium chloride (CaCl2) and bismuth oxide (Bi2O3) or zinc oxide (ZnO) additives on odontoblast cell response to CAC. Methodology: MDPC-23 cells were exposed for up to 14 d: 1) CAC with 2.8% CaCl2 and 25% ZnO (CACz); 2) CAC with 2.8% CaCl2 and 25% Bi2O3 (CACb); 3) CAC with 10% CaCl2 and 25% Bi2O3 (CACb+); or 4) mineral trioxide aggregate (MTA), placed on inserts. Non-exposed cultures served as control. Cell morphology, cell viability, gene expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and dentin matrix protein 1 (DMP-1), ALP activity, and extracellular matrix mineralization were evaluated. Data were compared using ANOVA (α=5%). Results: Lower cell density was detected only for MTA and CACb+ compared with Control, with areas showing reduced cell spreading. Cell viability was similar among groups at days one and three (p>0.05). CACb+ and MTA showed the lowest cell viability values at day seven (p>0.05). CACb and CACb+ promoted higher ALP and BSP expression compared with CACz (p<0.05); despite that, all cements supported ALP activity. Matrix mineralization were enhanced in CACb+ and MTA. Conclusion: In conclusion, CAC with Bi2O3, but not with ZnO, supported the expression of odontoblastic phenotype, but only the composition with 10% CaCl2 promoted mineralized matrix formation, rendering it suitable for dentin-pulp complex repair. (AU)

FAPESP's process: 10/50936-4 - The effects of aluminate cement preparations on the progression of osteogenic cell cultures and on undifferentiated mesenchymal pulp cells
Grantee:Paulo Tambasco de Oliveira
Support type: Regular Research Grants
FAPESP's process: 16/11461-7 - Odontoblast-like cell response to calcium aluminate cement
Grantee:Nadyne Saab Messias
Support type: Scholarships in Brazil - Scientific Initiation