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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Tissue Proteome Signatures Associated with Five Grades of Prostate Cancer and Benign Prostatic Hyperplasia

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Kawahara, Rebeca [1, 2] ; Recuero, Saulo [3] ; Nogueira, Fabio C. S. [4] ; Domont, Gilberto B. [4] ; Leite, Katia R. M. [3] ; Srougi, Miguel [3] ; Thaysen-Andersen, Morten [2] ; Palmisano, Giuseppe [1]
Total Authors: 8
[1] Univ Sao Paulo, Dept Parasitol, Inst Ciencias Biomed, BR-05508000 Sao Paulo - Brazil
[2] Macquarie Univ, Dept Mol Sci, Sydney, NSW 2109 - Australia
[3] Univ Sao Paulo, LIM55, Fac Med, Lab Invest Med Disciplina Urol, BR-01246903 Sao Paulo - Brazil
[4] Univ Fed Rio De Janeiro, Dept Bioquim, Inst Quim, BR-21941909 Rio De Janeiro - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PROTEOMICS; v. 19, n. 21-22, SI NOV 2019.
Web of Science Citations: 0

The histology-based Gleason score (GS) of prostate cancer (PCa) tissue biopsy is the most accurate predictor of disease aggressiveness and an important measure to guide treatment strategies and patient management. The variability associated with PCa tumor sampling and the subjective determination of the GS are challenges that limit accurate diagnostication and prognostication. Thus, novel molecular signatures are needed to distinguish between indolent and aggressive forms of PCa for better patient management and outcomes. Herein, label-free LC-MS/MS proteomics is used to profile the proteome of 50 PCa tissues spanning five grade groups (n = 10 per group) relative to tissues from individuals with benign prostatic hyperplasia (BPH). Over 2000 proteins are identified albeit at different levels between and within the patient groups, revealing biological processes associated with specific grades. A panel of 11 prostate-derived proteins including IGKV3D-20, RNASET2, TACC2, ANXA7, LMOD1, PRCP, GYG1, NDUFV1, H1FX, APOBEC3C, and CTSZ display the potential to stratify patients from low and high PCa grade groups. Parallel reaction monitoring of the same sample cohort validate the differential expression of LMOD1, GYG1, IGKV3D-20, and RNASET2. The four proteins associated with low and high PCa grades reported here warrant further exploration as candidate biomarkers for PCa aggressiveness. (AU)

FAPESP's process: 18/15549-1 - Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications
Grantee:Giuseppe Palmisano
Support type: Research Grants - Young Investigators Grants - Phase 2
Grantee:Rebeca Kawahara Sakuma
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/18257-1 - Multi-user equipment approved in grant 14/06863-3: HPLC system configured for analysis of carbohydrates, amino acidis, peptides and glycoproteins
Grantee:Giuseppe Palmisano
Support type: Multi-user Equipment Program
FAPESP's process: 17/03010-8 - Site-specific characterization of N- and O-linked glycosylation in prostate cancer tissues as molecular signature for disease progression
Grantee:Rebeca Kawahara Sakuma
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications
Grantee:Giuseppe Palmisano
Support type: Research Grants - Young Investigators Grants