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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Propargylglycine decreases neuro-immune interaction inducing pain response in temporomandibular joint inflammation model

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Author(s):
Garattini, Emanuela G. [1] ; Santos, Bruna M. [2] ; Ferrari, Daniele P. [1] ; Capel, Camila P. [1] ; Francescato, Heloisa D. C. [2] ; Coimbra, Terezila M. [2] ; Leite-Panissi, Christie R. A. [3] ; Branco, Luiz G. S. [2, 1] ; Nascimento, Glauce C. [2, 1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Basic & Oral Biol, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Physiol, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Sch Philosophy Sci & Literature Ribeirao Preto, Psychobiol Grad Program, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: NITRIC OXIDE-BIOLOGY AND CHEMISTRY; v. 93, p. 90-101, DEC 1 2019.
Web of Science Citations: 0
Abstract

The mechanisms underlying temporomandibular disorders following orofacial pain remain unclear. Hydrogen sulfide (H2S), a newly identified gasotransmitter, has been reported to modulate inflammation. Cystathionine gamma-lyase (CSE) is responsible for the systemical production of H2S, which exerts both pro- and antinociceptive effects through inflammation. In the current study, we investigated whether the endogenous H2S production pathway contributes to arousal and maintenance of orofacial inflammatory pain, through the investigation of the effects of a CSE inhibitor, propargyglycine (PAG), in a rat CFA (Complete Freund Adjuvant)-induced temporomandibular inflammation model to mimic persistent pain in the orofacial region. For this, rats received either CFA or saline in the temporomandibular joints (TMJs), and after 3 or 14 days, they received a single injection of PAG or saline and were evaluated for nociception with the von Frey and formalin test. Also, pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) were analyzed in TMJs and trigeminal ganglion (TG). In this last one, glial cells reactivity was also verified. Endogenous H2S production rate were measured in both, TMJ and TG. Our results indicated decreased allodynia and hyperalgesic responses in rats submitted to CFA after injection of PAG. Moreover, PAG inhibited leucocyte migration to temporomandibular synovial fluid after 3 and 14 days of inflammation. PAG was able to reduce levels of CBS, CSE, TNF-alpha, and IL-1 beta in the TMJ and TG, after 13 days of CFA injection. The observed increased activation of glial cells in the trigeminal ganglia on the 14th day of inflammation can be prevented by the highest dose of PAG. Finally, CBS and CSE expression, and endogenous H2S production rate in the TMJ and TG was found higher in rats with persistent temporomandibular inflammation compared to rats injected with saline and PAG was able to prevent this elevation. Our results elucidated the molecular mechanisms by which H2S exerts its pro-inflammatory and pro-nociceptive role in the orofacial region by alterations in both local tissue and TG. (AU)

FAPESP's process: 15/03053-3 - Evaluation of medial pterygoid muscle and brain structures related to orofacial nociception and emotional behavior in an animal model of chronic stress and masticatory hypofunction
Grantee:Glauce Crivelaro Do Nascimento
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/03645-3 - Role of hydrogen sulfide in orofacial allodynia modulation in an experimental model of arterial hypertension
Grantee:Camila Porto Capel
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/09364-3 - Role of endogenous preoptic hydrogen sulfide modulating LPS tolerance
Grantee:Bruna Maitan Santos
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/17681-9 - Pathophysiological changes during systemic inflammation
Grantee:Luiz Guilherme de Siqueira Branco
Support type: Research Projects - Thematic Grants