Electrochemical Behavior of Cytochrome C Immobilized in a Magnetically Induced Mesoporous Framework

Author(s)

Melo, Antonio F. A. A. ^{[1, 2, 3]} ; Sedenho, Graziela C. ^[1] ; Osica, Izabela ^{[4, 3]} ; Ariga, Katsuhiko ^{[5, 3]} ; Crespilho, Frank N. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Chem IQSC, BR-13560970 Sao Carlos, SP - Brazil

^[2] Fed Inst Educ Sci & Technol Piaui, BR-64000040 Teresina, PI - Brazil

^[3] NIMS, World Premier Int WPI Res Ctr Mat Nanoarchitecton, 1-1 Namiki, Tsukuba, Ibaraki 3050044 - Japan

^[4] Warsaw Univ Technol, Fac Mat Sci & Engn, Woloska 141, PL-02507 Warsaw - Poland

^[5] Univ Tokyo, Grad Sch Frontier Sci, Dept Adv Mat Sci, 5-1-5 Kashiwanoha, Kashiwa, Chiba 2778561 - Japan

• Document type: Journal article
• Source: CHEMELECTROCHEM; v. 6, n. 23, p. 5802-5809, DEC 2 2019.
• Web of Science Citations: 0

Abstract

Protein immobilization on electrode surfaces remains a technological challenge and is decisive in enhancing biological properties with simplified operational steps. In view of proposing a new direction for protein immobilization, we report the development of a mesoporous framework resulting from the magnetically induced assembly of magnetite nanoparticles decorated with near-monodisperse gold nanoparticles onto an indium-tin-oxide electrode. With an electrochemically active surface area of about 72 cm(2) and pores with an average size of 31 +/- 1 nm, this magnetically induced mesoporous framework is suitable for the immobilization of small redox proteins. As a proof-of-concept, we show the electrochemical process of cytochrome c, where the mesoporous electrode provided a fourfold enhancement for the faradaic currents in comparison to standard polycrystalline gold electrodes. (AU)

• FAPESP's process: 16/25806-6 - Interaction between biomolecules and nanostructures: electrochemistry, interfaces and surfaces
• Grantee: Ayaz Hassan
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/22973-6 - Development of Microbial Fuel Biocells for Bioproduction and Oxidation of Ethanol
• Grantee: Graziela Cristina Sedenho
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/01541-0 - Development of bioanodes composed by glucose oxidase and fructose dehydrogenase and its recombinant structures for aplication in prototype of biofuel cell: power generation from biomass (sugar cane)
• Grantee: Frank Nelson Crespilho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/04663-4 - Enzymatic bioelectrodes applied in biofuel cells: experimental and simulation
• Grantee: Frank Nelson Crespilho
• Support Opportunities: Regular Research Grants