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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Downregulation of PHYTOCHROME-INTERACTING FACTOR 4 Influences Plant Development and Fruit Production

Full text
Author(s):
Rosado, Daniele [1] ; Trench, Bruna [1] ; Bianchetti, Ricardo [1] ; Zuccarelli, Rafael [1] ; Rodrigues Alves, Frederico Rocha [1] ; Purgatto, Eduardo [2] ; Segal Floh, Eny Iochevet [1] ; Silveira Nogueira, Fabio Tebaldi [3] ; Freschi, Luciano [1] ; Rossi, Magdalena [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Plant Physiology; v. 181, n. 3, p. 1360-1370, NOV 2019.
Web of Science Citations: 1
Abstract

Plant development is highly dependent on the ability to perceive and cope with environmental changes. In this context, PIF proteins are key players in the cellular hub controlling responses to fluctuating light and temperature conditions. Reports in various plant species show that manipulation of the PIF4 level affects important agronomical traits. In tomato (Solanum lycopersicum), SlPIF1a and SlPIF3 regulate fruit nutraceutical composition. However, the wider role of this protein family, and the potential of their manipulation for the improvement of other traits, has not been explored. Here we report the effects of constitutive silencing of tomato SlPIF4 on whole-plant physiology and development. Ripening anticipation and higher carotenoid levels observed in SlPIF4-silenced fruits revealed a redundant role of SlPIF4 in the accumulation of nutraceutical compounds. Furthermore, silencing triggered a significant reduction in plant size, flowering, fruit yield, and fruit size. This phenotype was most likely caused by reduced auxin levels and altered carbon partitioning. Impaired thermomorphogenesis and delayed leaf senescence were also observed in silenced plants, highlighting the functional conservation of PIF4 homologs in angiosperms. Overall, this work improves our understanding of the role of PIF proteins-and light signaling-in metabolic and developmental processes that affect yield and composition of fleshy fruits. (AU)

FAPESP's process: 16/01128-9 - Light and hormonal regulation of nutritional quality in Solanum lycopersicum
Grantee:Maria Magdalena Rossi
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/02033-1 - Manipulation of nitric oxide levels and its impacts on tomato fruit physiology, hormonal balance and nutritional quality
Grantee:Rafael Zuccarelli
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/14658-3 - Functional characterization of PIF proteins (Phytochrome-Interacting Factors) in tomato, Solanum lycopersicum
Grantee:Daniele Silva Pereira Rosado
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/04924-0 - Manipulating phytochrome levels in tomato fruits (Solanum lycopersicum L.) and its impacts on plastid development, physiology and nutritional composition
Grantee:Frederico Rocha Rodrigues Alves
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/16764-8 - Phenotypic characterization of tomato plants (Solanum lycopersicum) knockdown for phytochrome interactions proteins (PIF1b and PIF4)
Grantee:Bruna Vasconcellos Trench Fonseca
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/24354-7 - Effects of phytochrome-dependent temperature perception in tomato fruit metabolism through epigenetic regulation
Grantee:Ricardo Ernesto Bianchetti
Support type: Scholarships in Brazil - Post-Doctorate